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Team:Korea U Seoul/Project/sub design
From 2014.igem.org
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<p>Our part is a “pilin A gene cluster”. Pilin A gene cluster is coding for pili A of Corynebacterium diphtheriae. It has four coding regions and one regulatory region. Regulatory region is its own promoter. Four coding regions are sortase A, enzyme that makes pili A and pilin spa A & B & C that are components of pili A. WIth these all regions, we can make pili A. And we are going to modifying this part to generate "Our Favorite Protein Whip", especially spa A<br /><br /> | <p>Our part is a “pilin A gene cluster”. Pilin A gene cluster is coding for pili A of Corynebacterium diphtheriae. It has four coding regions and one regulatory region. Regulatory region is its own promoter. Four coding regions are sortase A, enzyme that makes pili A and pilin spa A & B & C that are components of pili A. WIth these all regions, we can make pili A. And we are going to modifying this part to generate "Our Favorite Protein Whip", especially spa A<br /><br /> | ||
| - | Because of internal restriction enzyme site in our part, we have to slice it to two parts. We can use pilin A gene cluster by cutting and fusing these two parts. Vector is pSB1C3 | + | Because of internal restriction enzyme site in our part, we have to slice it to two parts. We can use pilin A gene cluster by cutting and fusing these two parts. Vector is pSB1C3<br /><br /> |
| + | BBa_K1377000 Link : <a href="http://parts.igem.org/Part:BBa_K1377000">http://parts.igem.org/Part:BBa_K1377000</a><br/> | ||
| + | BBa_K1377001 Link : <a href="http://parts.igem.org/Part:BBa_K1377001">http://parts.igem.org/Part:BBa_K1377001</a><br/> | ||
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<img src="https://static.igem.org/mediawiki/2014/2/2e/Part1.png"><br/><br/> | <img src="https://static.igem.org/mediawiki/2014/2/2e/Part1.png"><br/><br/> | ||
Revision as of 04:36, 17 October 2014
Our part is a “pilin A gene cluster”. Pilin A gene cluster is coding for pili A of Corynebacterium diphtheriae. It has four coding regions and one regulatory region. Regulatory region is its own promoter. Four coding regions are sortase A, enzyme that makes pili A and pilin spa A & B & C that are components of pili A. WIth these all regions, we can make pili A. And we are going to modifying this part to generate "Our Favorite Protein Whip", especially spa A
Because of internal restriction enzyme site in our part, we have to slice it to two parts. We can use pilin A gene cluster by cutting and fusing these two parts. Vector is pSB1C3
BBa_K1377000 Link : http://parts.igem.org/Part:BBa_K1377000
BBa_K1377001 Link : http://parts.igem.org/Part:BBa_K1377001
| Name | Type | Description | Length | Designer |
|---|---|---|---|---|
| BBa_K1377000 | Device |
promoter of pili A gene cluster + spaA front |
1137bp | Jade Minseob Yoo |
| BBa_K1377001 | Device | spaA backward + sortase A + spaB + spaC | 7772bp | Jade Minseob Yoo |
