Team:UIUC Illinois/Software/Evolvalvability

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Revision as of 04:29, 17 October 2014

Do you fear that your plasmid will be lost by mutation?

Here is our EvoAnalyzer software that will predict mutational tolerance of plasmid

in K12 E.Coli strain!!

Description: The basis is how evolution depends on both mutational rate on a single organism and the selection of organisms within a group in terms of who receives certain mutations. There are many steps that must be accounted in order to proximate more accurately. This is why we must be modular in our approach. As a preliminary step, and due to the specificity of the data we will be using, we specifically choose a common strain of E. coli which is K12.

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 <p><b>Description:</b>
-Currently there are few websites that give you only cut sites, however we heard that many synthetic biologists struggle with obtaining optimized enzymes
+The basis is how evolution depends on both mutational rate on a single organism and the selection of organisms within a group in terms of who receives certain mutations. There are many steps that must be accounted in order to proximate more accurately. This is why we must be modular in our approach. As a preliminary step, and due to the specificity of the data we will be using, we specifically choose a common strain of E. coli which is K12.
-Cutsultant is a python script created to expedite the process of finding restriction enzymes that produce the most optimal digestions. Using Biopython's restriction enzyme package, as well as user-inputted sequences and available restriction enzymes Cutsultant can use one or two sequences (typically a plasmid and/or a plasmid with an insert) and generate lists of enzymes that produce an optimal cut based on a a few parameters, such as number of cuts, minimum band length, maximum band length, and differentiability of bands. </p>
+</p>