Team:ITB Indonesia/RepMod

From 2014.igem.org

(Difference between revisions)
Line 231: Line 231:
<p>Reporter module is designed to detect the existence of cytoplasmic stress by naked eye. One of the cytoplasmic stresses is the formation of inclusion body due to overexpression of LC Cutinase in degradation module. The overexpression of the protein may result in an inefficient protein folding where not 100% proteins are useable and probably, the misfolded protein will create an aggregate known as inclusion body that decreasing the system efficiency.</p>
<p>Reporter module is designed to detect the existence of cytoplasmic stress by naked eye. One of the cytoplasmic stresses is the formation of inclusion body due to overexpression of LC Cutinase in degradation module. The overexpression of the protein may result in an inefficient protein folding where not 100% proteins are useable and probably, the misfolded protein will create an aggregate known as inclusion body that decreasing the system efficiency.</p>
<p>Inclusion body inducible promoter (PibpAB) then will detect the formation of inclusion body and report it by expressing amilCP, a blue fluorescent protein that detectable by naked eyes, make it easier to analyze whether the stress level of the cell is in the limit or beyond the limit.</p>
<p>Inclusion body inducible promoter (PibpAB) then will detect the formation of inclusion body and report it by expressing amilCP, a blue fluorescent protein that detectable by naked eyes, make it easier to analyze whether the stress level of the cell is in the limit or beyond the limit.</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2014/b/ba/Reporter-module.jpg">
 +
 +
<br>
 +
<br>
 +
 +
<div id="sponsor"></div>
 +
</div>
 +
</div>
 +
</body>
 +
</html>
 +
<html>
 +
<head>
 +
<title></title>
 +
<style>
 +
body{
 +
background-image: url(https://static.igem.org/mediawiki/2014/4/47/Itb-bg.jpg);
 +
background-repeat: repeat;
 +
}#logo-igem{width:160px;height:100px;position:absolute;right:28px;top:33px;background-color:orange;cursor:pointer;opacity:0.01}
 +
 +
 +
#top-section{width:1010px;height:47px;background-color:#FFFFFF;}
 +
 +
#menubar{top:10px;padding-left:25px;padding-right:25px;}
 +
 +
#menubar ul li a{background-color:#FFFFFF;color:black;}
 +
#menubar a:hover{text-decoration:underline;}
 +
#menubar.left-menu.noprint{background-color:#FFFFFF;}
 +
 +
#menubar ul li:nth-child(1){background-color:#FFFFFF;color:black;}
 +
#menubar ul li:nth-child(2){background-color:#FFFFFF;color:black;}
 +
#menubar ul li:nth-child(3){background-color:#FFFFFF;color:black;}
 +
#menubar ul li:nth-child(4){background-color:#FFFFFF;color:black;}
 +
#menubar ul li:nth-child(5){background-color:#FFFFFF;color:black;}
 +
#menubar ul li:nth-child(6){background-color:#FFFFFF;color:black;}
 +
#menubar ul li:nth-child(7){visibility:hidden;}
 +
 +
#p-logo img{display:none;}
 +
 +
#search-controls{display:none;}
 +
 +
.firstHeading{display:none;}
 +
#content{width:1000px;}
 +
#footer-box{display:none;}
 +
 +
#putih{
 +
width: 1000px;
 +
margin: 0 auto;
 +
background-color: #FFFFFF;
 +
}
 +
 +
#center{
 +
width: 960px;
 +
margin: 0 auto;
 +
}
 +
 +
 +
/*****    Start Header    *****/
 +
#logo{
 +
width: 960px;
 +
height: 100px;
 +
background-image: url(https://static.igem.org/mediawiki/2014/3/3e/Itb-logo.jpg);
 +
/*background-color: #FFFFAA;*/
 +
/*position: absolute;
 +
top: 0;*/
 +
}
 +
 +
#header{
 +
/*margin-top: 10px;*/
 +
width: 960px;
 +
height: 200px;
 +
background-image: url(https://static.igem.org/mediawiki/2014/5/56/Itb-judul.jpg);
 +
}
 +
/*****    End Header    *****/
 +
 +
 +
/*****    Start Nav    *****/
 +
#blockNav{
 +
width: 960px;
 +
height: 48px;
 +
background-color: #38AF89;
 +
}
 +
 +
ul {
 +
  text-align: left;
 +
  display: inline;
 +
  margin: 0;
 +
  padding: 15px 4px 17px 0;
 +
  list-style: none;
 +
  /*-webkit-box-shadow: 0 0 5px rgba(0, 0, 0, 0.15);
 +
  -moz-box-shadow: 0 0 5px rgba(0, 0, 0, 0.15);
 +
  box-shadow: 0 0 5px rgba(0, 0, 0, 0.15);*/
 +
}
 +
ul li {
 +
  font: bold 12px/18px sans-serif;
 +
  /*font-family: source-code-pro, sans-serif;*/
 +
  color: #FFFFFF;
 +
  display: inline-block;
 +
  margin-right: -4px;
 +
  position: relative;
 +
  padding: 15px 20px;
 +
  background: #38AF89;
 +
  cursor: pointer;
 +
  -webkit-transition: all 0.2s;
 +
  -moz-transition: all 0.2s;
 +
  -ms-transition: all 0.2s;
 +
  -o-transition: all 0.2s;
 +
  transition: all 0.2s;
 +
}
 +
ul li:hover {
 +
  background: #2E9876;
 +
  color: #FFFFFF;
 +
}
 +
ul li ul {
 +
  padding: 0;
 +
  position: absolute;
 +
  top: 48px;
 +
  left: 0;
 +
  width: 180px;
 +
  -webkit-box-shadow: none;
 +
  -moz-box-shadow: none;
 +
  box-shadow: none;
 +
  display: none;
 +
  opacity: 0;
 +
  visibility: hidden;
 +
  -webkit-transiton: opacity 0.2s;
 +
  -moz-transition: opacity 0.2s;
 +
  -ms-transition: opacity 0.2s;
 +
  -o-transition: opacity 0.2s;
 +
  -transition: opacity 0.2s;
 +
}
 +
ul li ul li{
 +
  background: #2E9876;
 +
  display: block;
 +
  color: #FFFFFF;
 +
  text-shadow: 0 -1px 0 #000;
 +
}
 +
ul li ul li:hover { background: #4CB795; }
 +
ul li:hover ul {
 +
  display: block;
 +
  opacity: 1;
 +
  visibility: visible;
 +
}
 +
 +
a:link {
 +
    text-decoration: none;
 +
    color: #FFFFFF;
 +
}
 +
 +
a:visited {
 +
    text-decoration: none;
 +
    color: #FFFFFF;
 +
}
 +
/*****    End Nav    *****/
 +
 +
 +
/*****    Start Content    *****/
 +
iframe{
 +
margin: 30px 0 30px 200px;
 +
}
 +
 +
#line{
 +
width: 960px;
 +
height: 1px;
 +
background-color: #BCBCB3;
 +
}
 +
/*****    End Content    *****/
 +
 +
/*****    Start Sponsor    *****/
 +
#sponsor{
 +
width: 960px;
 +
height: 200px;
 +
background-image: url(https://static.igem.org/mediawiki/2014/3/36/Itb-sponsor.jpg);
 +
}
 +
/*****    End Sponsor    *****/
 +
 +
 +
</style>
 +
<script src="//use.edgefonts.net/source-code-pro.js"></script>
 +
</head>
 +
<body>
 +
<div id="putih"><div id="logo-igem" onclick="window.location.href='https://2014.igem.org/'"></div>
 +
<div id="center">
 +
<div id="logo"></div>
 +
 +
<div id="header"></div>
 +
 +
<div id="blockNav">
 +
<ul>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia">HOME</a></li>
 +
<li>TEAM
 +
<ul>
 +
<li><a href="https://igem.org/Team.cgi?id=1387" >OFFICIAL TEAM PROFILE</a></li>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Team">TEAM MEMBER</a></li>
 +
</ul>
 +
</li>
 +
<li>PROJECT
 +
<ul>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Project">OVERVIEW</a></li>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/DegMod">DEGRADATION MODULE</a></li>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/ConMod">CONVERTION MODULE</a></li>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/RepMod">REPORTER MODULE</a></li>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/SelfMod">SELF REGULATORY MODULE</a></li>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/FutureSystem">FUTURE SYSTEM</a></li>
 +
    </ul>
 +
</li>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Modeling">MODELING</a></li>
 +
<li>WETLAB
 +
<ul>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/protocol">PROTOCOL</a></li>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Parts">PARTS</a></li>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Attributions">ATTRIBUTIONS</a></li>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Safety">SAFETY</a></li>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Data">DATA</a></li>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/ProteinModel">PROTEIN MODEL</a></li>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Achievement">ACHIEVEMENT</a></li>
 +
</ul>
 +
</li>
 +
<li>NOTEBOOK
 +
<ul>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/nb-wetlab">WETLAB</a></li>
 +
</ul>
 +
</li>
 +
<li>HUMAN PRACTICE
 +
<ul>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Unpas">INTRO SYNBIO TO UNPAS</a></li>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/SynBio">SYNBIO CLASS</a></li>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/SynbiGreen">SYNBIGREEN SURVEY</a></li>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Medsos">SOCIAL MEDIA</a></li>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/OnMedia">MEDIA</a></li>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Biosafety">BIOSAFETY SEMINAR</a></li>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/MeetUp">INDONESIA TEAMS MEET UP</a></li>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Kids">FUN WITH KIDS</a></li>
 +
<li><a href="https://2014.igem.org/Team:ITB_Indonesia/UPI">SHARING SYNBIO IN UPI</a></li>
 +
</ul>
 +
</li>
 +
</ul>
 +
</div>
 +
 +
<br>
 +
<h1>Reporter Module</h1>
 +
<p align="justify">Reporter module is designed to detect the existence of cytoplasmic stress by naked eye. One of the cytoplasmic stresses is the formation of inclusion body due to overexpression of LC Cutinase in degradation module. The overexpression of the protein may result in an inefficient protein folding where not 100% proteins are useable and probably, the misfolded protein will create an aggregate known as inclusion body that decreasing the system efficiency.</p>
 +
<p align="justify">Inclusion body inducible promoter (PibpAB) then will detect the formation of inclusion body and report it by expressing amilCP, a blue fluorescent protein that detectable by naked eyes, make it easier to analyze whether the stress level of the cell is in the limit or beyond the limit.</p>
<img src="https://static.igem.org/mediawiki/2014/b/ba/Reporter-module.jpg">
<img src="https://static.igem.org/mediawiki/2014/b/ba/Reporter-module.jpg">

Revision as of 03:40, 17 October 2014


Reporter Module

Reporter module is designed to detect the existence of cytoplasmic stress by naked eye. One of the cytoplasmic stresses is the formation of inclusion body due to overexpression of LC Cutinase in degradation module. The overexpression of the protein may result in an inefficient protein folding where not 100% proteins are useable and probably, the misfolded protein will create an aggregate known as inclusion body that decreasing the system efficiency.

Inclusion body inducible promoter (PibpAB) then will detect the formation of inclusion body and report it by expressing amilCP, a blue fluorescent protein that detectable by naked eyes, make it easier to analyze whether the stress level of the cell is in the limit or beyond the limit.




Reporter Module

Reporter module is designed to detect the existence of cytoplasmic stress by naked eye. One of the cytoplasmic stresses is the formation of inclusion body due to overexpression of LC Cutinase in degradation module. The overexpression of the protein may result in an inefficient protein folding where not 100% proteins are useable and probably, the misfolded protein will create an aggregate known as inclusion body that decreasing the system efficiency.

Inclusion body inducible promoter (PibpAB) then will detect the formation of inclusion body and report it by expressing amilCP, a blue fluorescent protein that detectable by naked eyes, make it easier to analyze whether the stress level of the cell is in the limit or beyond the limit.



Retrieved from "http://2014.igem.org/Team:ITB_Indonesia/RepMod"