Team:WPI-Worcester

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</head>
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<!--Float Bar-->
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<body class="basiclayout">
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<div id="floatbar">
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<div id="bu-wellesley_wiki_content"></div>
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<a href="https://2014.igem.org/Main_Page"> <img src= "https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" align="right" width="55px"/></a>
+
 
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<a href="https://2014.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" alt="iGEM 2014 main page" style="position: absolute; top: 10px; right: 10px;"width="50"></a>
</div>
</div>
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<body>
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<p  style="text-align:center;"><a href="https://2014.igem.org/Team:WPI-Worcester"><img src="https://static.igem.org/mediawiki/2014/3/3f/Wpibanner.png" width="800px" length="200px"></a></p>
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<div id="toptext">
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<p>This wiki is under heavy construction.  It works best on Chrome, and barely at all on Internet Explorer.  Look under the Notebook tab for our experiments or scroll down for some other stuff.</p>
+
<div id="menuOverlay">
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<a href="https://2014.igem.org/Team:WPI-Worcester/Tour1">Click here for a quick click-through tour!</a>
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<ul id="nav">  
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</div>
+
<li> <a href="#"><center><img src="https://static.igem.org/mediawiki/2014/6/64/WPI_Team_Link.png"/></center> <p>Team</p></a>
-
<div id="page">
+
<ul>
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<div id="shift">
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<li><a href="https://2014.igem.org/Team:WPI-Worcester/Team">Bios</a></li>
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<img src="https://static.igem.org/mediawiki/2014/c/c8/WPI_Team_Logo.png" width="300px"/>
+
</ul>
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</div>
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</li>
-
<div id="title">
+
-
<img src="https://static.igem.org/mediawiki/2014/9/9a/WPI_Title.png" width="800px"/>
+
<li><a href="#"><center><img src="https://static.igem.org/mediawiki/2014/4/41/WPI_Project_Link.png"/></center><p>Project</p></a>  
-
</div>
+
<ul>  
-
<div id="menubar1">
+
    <li><a href="https://2014.igem.org/Team:WPI-Worcester/Overview">Project Overview and Abstract</a></li>  
-
<div class="menuslide">
+
            <li><a href="https://2014.igem.org/Team:WPI-Worcester/Background">Background</a></li>
-
<a href="https://2014.igem.org/Team:WPI-Worcester"> Home </a>
+
            <li><a href="https://2014.igem.org/Team:WPI-Worcester/Motivation">Motivation</a></li>
-
<p><img src="https://static.igem.org/mediawiki/2014/f/f8/WPI_Home_Link.png"/></p>
+
    <li><a href="https://2014.igem.org/Team:WPI-Worcester/Future-Applications">Future Applications</a></li>
-
<div class="dropdown">
+
 
-
<a href="https://2014.igem.org/Team:WPI-Worcester/Tour1"> Tour</a>
+
</ul>
-
<a href="https://2014.igem.org/Team:WPI-Worcester/Project/Accomplishments"> Accomplishments </a>
+
</li>
-
</div>
+
-
</div>
+
<li><a href="#"><center><img src="https://static.igem.org/mediawiki/2014/f/f1/Accomplishments_RealLink.png"/></center><p>Accomplishments</p></a>  
-
<div class="menuslide">
+
<ul>  
-
<a href="https://2014.igem.org/Team:WPI-Worcester/Team"> Team </a>
+
<li><a href="https://2014.igem.org/Team:WPI-Worcester/Our-Construct">Our Construct</a></li>
-
<p><img src="https://static.igem.org/mediawiki/2014/6/64/WPI_Team_Link.png"/></p>
+
<li><a href="https://2014.igem.org/Team:WPI-Worcester/Proof-of-Principle">Proof of Principle</a></li>
-
<div class="dropdown">
+
<li><a href="https://2014.igem.org/Team:WPI-Worcester/ATF1">Biobrick Characterization</a></li>
-
<a href="https://igem.org/Team.cgi?id=1423"> Official Page</a>
+
<li><a href="https://2014.igem.org/Team:WPI-Worcester/Biobricks">Biobricks</a></li>  
-
<a href="https://2014.igem.org/Team:WPI-Worcester/Team"> Bios</a>
+
<li><a href="https://2014.igem.org/Team:WPI-Worcester/Medal-Fulfillment">Medal Fulfillment</a></li>
-
<a href="https://2014.igem.org/Team:WPI-Worcester/Attributions"> Attributions</a>
+
 
-
</div>
+
</ul>
-
</div>
+
</li>
-
<div class="menuslide">
+
 
-
<a href="https://2014.igem.org/Team:WPI-Worcester/Project"> Projects </a>
+
<li><a href="#"><center><img src="https://static.igem.org/mediawiki/2014/7/7e/WPI_Notes_Link.png"/></center><p>Notebook</p></a>
-
<p><img src="https://static.igem.org/mediawiki/2014/4/41/WPI_Project_Link.png"/></p>
+
<ul>
-
<div class="dropdown">
+
<li><a href="https://2014.igem.org/Team:WPI-Worcester/Notebook">Weekly Log</a></li>
-
<a href="https://2014.igem.org/Team:WPI-Worcester/Project"> Agglutination</a>
+
<li><a href="https://2014.igem.org/Team:WPI-Worcester/Protocols">Protocols</a></li>
-
<a href="https://2014.igem.org/Team:WPI-Worcester/Project/Interlab"> Interlab Study</a>
+
-
<a href="https://2014.igem.org/Team:WPI-Worcester/Project/ATF1"> Quantifying ATF1 </a>
+
</ul>
-
</div>
+
</li>
-
</div>
+
-
<div class="menuslide">
+
 
-
<a href="https://2014.igem.org/Team:WPI-Worcester/Notebook"> Notebook </a>
+
<li><a href="#"><center><img src="https://static.igem.org/mediawiki/2014/d/d4/WPI_Safety_Link.png"/></center><p>Practices</p></a>  
-
<p><img src="https://static.igem.org/mediawiki/2014/7/7e/WPI_Notes_Link.png"/></p>
+
<ul>
-
<div class="dropdown">
+
            <li><a href="https://2014.igem.org/Team:WPI-Worcester/Survey">Survey</a></li>
-
<a href="https://2014.igem.org/Team:WPI-Worcester/Notebook"> Weekly Updates</a>
+
            <li><a href="https://2014.igem.org/Team:WPI-Worcester/Outreach">Outreach</a></li>
-
<a href="https://2014.igem.org/Team:WPI-Worcester/Notebook/Biobricks"> Biobricks</a>
+
<li><a href="https://2014.igem.org/Team:WPI-Worcester/Collaborations">Collaborations</a></li>
-
</div>
+
<li><a href="https://2014.igem.org/Team:WPI-Worcester/Interlab">Interlab Study</a></li>
-
</div>
+
  <li><a href="https://2014.igem.org/Team:WPI-Worcester/Safety">Safety</a></li>
-
<div class="menuslide">
+
            <li><a href="https://2014.igem.org/Team:WPI-Worcester/Beyond-the-Bench">Beyond the Bench</a></li>
-
<a href="https://2014.igem.org/Team:WPI-Worcester/Practices">Practices </a>
+
 
-
<p><img src="https://static.igem.org/mediawiki/2014/d/d4/WPI_Safety_Link.png"/></p>
+
        </ul>
-
<div class="dropdown">
+
 
-
<a href="https://2014.igem.org/Team:WPI-Worcester/Practices/Safety"> Safety </a>
+
 
-
<a href="https://2014.igem.org/Team:WPI-Worcester/Practices/Collaborations"> Collaborations </a>
+
<li><a href="#"><center><img src="https://static.igem.org/mediawiki/2014/e/e9/Acknowledgements_Link.png"/></center><p>Acknowledgements</p></a>
-
<a href="https://2014.igem.org/Team:WPI-Worcester/Practices/Acknowledgements"> Acknowledgements </a>
+
<ul>
-
</div>
+
<li><a href="https://2014.igem.org/Team:WPI-Worcester/Sponsors">Sponsors</a></li>
-
</div>
+
<li><a href="https://2014.igem.org/Team:WPI-Worcester/Attributions">Attributions</a></li>
-
</div>
+
-
</div>
+
</ul>
-
<div id="maintext">
+
</li>
-
<div class="textitem">
+
</li>
-
<h1>Our Project</h1>
+
</ul>
-
<div class="bigtext">
+
-
Just this year, the British government offered a 10 million pound prize, known as the Longitude Prize,
+
-
to incentivize research for solving antibiotic resistance, which was deemed one of the most pressing issues
+
-
of our time. The rise of antibiotic resistant strains of bacteria can be partly attributed to the over-prescription
+
-
of antibiotics. The overuse of antibiotics creates selective pressure resulting in the propagation of antibiotic
+
-
resistant genotypes. The farming industry is also a culprit in the over prescription of antibiotics. In fact,
+
-
livestock are administered more than 3 times the amount of antibiotics that are prescribed to humans.
+
-
In order to combat the excessive prescription of antibiotics, an effective and cheap method of diagnosing disease is
+
-
essential. Our team is creating a construct using a protein anchoring motif from Bacillus anthracis called BclA
+
-
that is capable of presenting an antigen from a disease on the surface of E.coli.  In order to test if an individual
+
-
is infected with a particular disease, the antigen to that disease will be localized to the E.coli cell surface using
+
-
BclA and then the patients' blood will be mixed with the transformed bacteria. The immune response in the individual would have
+
-
already created antibodies in the blood to combat the body's infection.  Thus, if the patients'
+
-
blood has the antibodies to the disease of interest, the antibodies will form a complex with the antigen presented on the
+
-
E.coli's surface, agglutinating and creating a visible clumping, indicating a positive diagnosis for the disease in the patient.
+
-
</div>
+
-
</div>
+
-
<div class="textitem">
+
-
<h1>A Picture Summary of our Project</h1>
+
-
<a href="https://2014.igem.org/Team:WPI-Worcester/Project">
+
-
<img src="https://static.igem.org/mediawiki/2014/b/b1/WPI_2014_Infographic3.png" height=300px />
+
-
</a>
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</div>
+
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<div class="textitem">
+
-
<h1>Gateway Labs</h1>
+
-
<div id="down">
+
-
<figure id="top">
+
-
<img src="http://i.imgur.com/iD2knlx.jpg" height=300px />
+
-
</figure>
+
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<figure id="pic2">
+
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<img src="http://i.imgur.com/GOsAvOI.jpg" height=300px />
+
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</figure>
+
-
<figure id="pic3">
+
-
<img src="http://i.imgur.com/jwGcKeB.jpg" height=300px />
+
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</figure>
+
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<figure id="pic4">
+
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<img src="http://i.imgur.com/uiMsH2C.jpg" height=300px />
+
-
</figure>
+
-
</div>
+
-
</div>
+
-
<div class="textitem">
+
-
<h1>An article about us</h1>
+
-
<a href="http://www.wpi.edu/news/20134/igemteam.html">
+
-
<img src="https://static.igem.org/mediawiki/2014/5/50/WPI_IGEM_students1.jpg" height=300px />
+
-
</a>
+
-
</div>
+
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<div class="textitem">
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<h1>The Interlab Study</h1>
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<h1>Women in Science Outreach</h1>
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<a href="https://2014.igem.org/Team:WPI-Worcester/About">About this wiki!</a>
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<h4>Ag-goat-ination:</h4><p><h8>Combating Antibiotic Resistance through Diagnostic Agglutination Assays</h8></p><p>Just this year, in a vote held for the Longitude Prize, antibiotic resistance was voted the most pressing issue of our time. One of the biggest causes of antibiotic resistance lies in the farming industry; more than 3 times the amount of antibiotics given to humans are administered to livestock. Over-prescription of antibiotics promotes resistance in both livestock pathogens and natural flora, which can be transmitted to humans through meat and other products.</p>
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<p>A cheap, quick, and reliable mechanism for detecting and identifying infection is one solution to this problem. We developed an E.coli based cell surface antigen expression system that can be utilized as a diagnostic tool for nearly any viral, bacterial, or fungal infection. The recombinant E.coli are utilized in a direct agglutination test using the host organism’s serum. Our team successfully demonstrated the feasibility of this diagnostic tool using a GFP antibody and antigen pair. </p>
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Revision as of 03:15, 17 October 2014

Team:WPI-Worcester - 2014.igem.org

 

Team:WPI-Worcester

From 2014.igem.org


Ag-goat-ination:

Combating Antibiotic Resistance through Diagnostic Agglutination Assays

Just this year, in a vote held for the Longitude Prize, antibiotic resistance was voted the most pressing issue of our time. One of the biggest causes of antibiotic resistance lies in the farming industry; more than 3 times the amount of antibiotics given to humans are administered to livestock. Over-prescription of antibiotics promotes resistance in both livestock pathogens and natural flora, which can be transmitted to humans through meat and other products.

A cheap, quick, and reliable mechanism for detecting and identifying infection is one solution to this problem. We developed an E.coli based cell surface antigen expression system that can be utilized as a diagnostic tool for nearly any viral, bacterial, or fungal infection. The recombinant E.coli are utilized in a direct agglutination test using the host organism’s serum. Our team successfully demonstrated the feasibility of this diagnostic tool using a GFP antibody and antigen pair.




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