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Team:Paris Saclay/Notebook/July/8
From 2014.igem.org
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| - | ==== | + | ====1 - Rehydration of the following biobricks:==== |
'''Sean''' | '''Sean''' | ||
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# Transfer contents of well into a 1.5 mL microcentrifuge tube. | # Transfer contents of well into a 1.5 mL microcentrifuge tube. | ||
| - | ==== | + | ====2 - Transformation in DH5α of the following biobricks:==== |
'''Mathieu & Maher''' | '''Mathieu & Maher''' | ||
* BBa B0015(CmR) | * BBa B0015(CmR) | ||
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NB : The protocol was modified. Instead of spreading 10-1 and 10-2 solutions of bacteria we took a non diluted solution and a 10-1 solution. | NB : The protocol was modified. Instead of spreading 10-1 and 10-2 solutions of bacteria we took a non diluted solution and a 10-1 solution. | ||
| - | ==== | + | ====3 - Plasmid DNA preparation:==== |
'''Romain & Sean''' | '''Romain & Sean''' | ||
Revision as of 09:13, 10 July 2014
Contents |
Tuesday 8th July
Lab work
1 - Rehydration of the following biobricks:
Sean
- BBa B0015(CmR)
- BBa J23119(CmR)
- BBa K731020(CmR)
- BBa K206000(CmR)
- BBa J04500(CmR)
- BBa J23100(AmpR)
- BBa J23106(AmpR)
- BBa J23114(AmpR)
Protocol:
- Pierce foil cover of well containing biobrick of interest.
- Add 10 μL of water in well.
- Transfer contents of well into a 1.5 mL microcentrifuge tube.
2 - Transformation in DH5α of the following biobricks:
Mathieu & Maher
- BBa B0015(CmR)
- BBa J23119(CmR)
- BBa K731020(CmR)
- BBa K206000(CmR)
- BBa J04500(CmR)
- BBa J23100(AmpR)
- BBa J23106(AmpR)
- BBa J23114(AmpR
- Control + : pUC18 (20ng)
- Control -
NB : The protocol was modified. Instead of spreading 10-1 and 10-2 solutions of bacteria we took a non diluted solution and a 10-1 solution.
3 - Plasmid DNA preparation:
Romain & Sean
Plasmids used:
- pJBEI-6409
E. coli strain used:
- XL1-Blue
