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Team:Paris Saclay/Notebook/July/9
From 2014.igem.org
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| - | = | + | =Wednesday 9th July= |
| - | == | + | ==Lab work== |
| - | ==== | + | ====1 - Plasmid DNA Extraction:==== |
'''Romain & Sean''' | '''Romain & Sean''' | ||
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[https://2013.igem.org/Team:Paris_Saclay/extraction Protocol] | [https://2013.igem.org/Team:Paris_Saclay/extraction Protocol] | ||
| - | ==== | + | ====2 - Electrophoresis:==== |
'''Arnaud & Romain & Sean''' | '''Arnaud & Romain & Sean''' | ||
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[https://2013.igem.org/Team:Paris_Saclay/gel Protocol] | [https://2013.igem.org/Team:Paris_Saclay/gel Protocol] | ||
| - | ==== | + | ====3 - New culture:==== |
'''Romain''' | '''Romain''' | ||
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4x 5ml LB + 20μl Chloramphenicol + a little of bacteria. We incubate cultures at 37°C. | 4x 5ml LB + 20μl Chloramphenicol + a little of bacteria. We incubate cultures at 37°C. | ||
| - | == | + | ==Human Pratices== |
Conference with the team to discuss about the project. The handled topics were: | Conference with the team to discuss about the project. The handled topics were: | ||
* Definition of Biobricks to make | * Definition of Biobricks to make | ||
Revision as of 09:12, 10 July 2014
Contents |
Wednesday 9th July
Lab work
1 - Plasmid DNA Extraction:
Romain & Sean
Plasmids used: DH5αZ1 P5B1C3 RBS amilCP Ter clone 9, A and B.
2 - Electrophoresis:
Arnaud & Romain & Sean
Plasmids used:
- DH5αZ1 P5B1C3 RBS amilCP Ter clone 9, A.
- DH5αZ1 P5B1C3 RBS amilCP Ter clone 9, B.
- XL1-Blue pCola-ges Kan
- pJBEI-6409 Cm
3 - New culture:
Romain
New liquid cultures of:
- FNR-RBS-amilCp-Ter DH5αZ1 P5B1C3 Cm
- BBa B0015 dble ter P5B1C3 Cm
4x 5ml LB + 20μl Chloramphenicol + a little of bacteria. We incubate cultures at 37°C.
Human Pratices
Conference with the team to discuss about the project. The handled topics were:
- Definition of Biobricks to make
- Planning of internships
- Definition of Biobricks to render
- Understanding the construction of consecutive genes
- Organisation and planning of experiences to do
- Sponsoring
- Wiki's progress
- Modelling's progress
People there:
- Instructors and advisors: Alice, Olivier, Solenne and Sylvie.
- Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
