Team:UANL Mty-Mexico/MathModel
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Revision as of 03:01, 17 October 2014
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Deterministic model Our genetic system is composed by two parts, each one placed in different vectors: the first in the plasmid 2-4B C/C, consists of the transcription factor cI (c0051) under the regulation of a promoter with a riboswitch ( J23100 and k115017 ) that can be activated or repressed depending on the temperature; we call this our “thermosensor”. We found this piece in the iGEM TUDelf-2008 Team and saw we could use it because of its specific translation temperature at 32ºC. The second part located in the vector 2-6B K/K consist of two genes -the insecticide protein Vip3Ca3 and the green fluorescent protein, (GFP); both are under the regulation of cI(c0051). The thermosensor is repressed when the temperature is below 32°C, it does not translate because the mRNA takes a secondary structure just as the image below and cannot enter the ribosome for translation; when the temperatures reaches a range between 32°C and 37°C, the thermosensor is activated, the mRNA unfolds into a straight structure that now have access to the ribosome, start translation, be passed to aminoacids and form a protein . cI (c0051) is a gene that constitutively represses the promoter r0051. Once our promoter isn't affected by the cI(c0051), the promoter is activated once the production of Vip3Ac3 and GFP started. This means that a range below 32°C to 37°C enables the production of the Vip and GFP. |