Team:Austin Texas/kit

From 2014.igem.org

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(Using The Kit)
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*'''pFRYC''' is the control plasmid and contains an IPTG-induced reporter system and a kanamycin resistance gene.  The reporter system is composed of RFP and sfGFP fused via a linker sequence between the two.
*'''pFRYC''' is the control plasmid and contains an IPTG-induced reporter system and a kanamycin resistance gene.  The reporter system is composed of RFP and sfGFP fused via a linker sequence between the two.
*'''pFRY''' is the experimental reporter plasmid and is nearly identical to pFRYC with the exception that its linker sequence contains an amber codon (star in '''Figure 2''') in the middle of the linker, whereas pFRYC contains a tyrosine codon in the same location.
*'''pFRY''' is the experimental reporter plasmid and is nearly identical to pFRYC with the exception that its linker sequence contains an amber codon (star in '''Figure 2''') in the middle of the linker, whereas pFRYC contains a tyrosine codon in the same location.
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==Using The Kit==
==Using The Kit==

Revision as of 02:49, 17 October 2014