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Team:UIUC Illinois/Notebook
From 2014.igem.org
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| - | + | PROJECT NOTES | |
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| - | NOTES | + | NOTES BY MONTH |
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| - | <h2> | + | <h2>Monday 9<sup>th</sup> June</h2> |
| - | <a href="https:// | + | <a href="https://2014.igem.org/Team:UIUC_Illinois/Project/Overview" target="_blank" class="tbnotelogo PSlogo"> ASDF </a> |
<div style="clear: both;"></div> | <div style="clear: both;"></div> | ||
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| - | + | Made LB broth using 10 grams of bacto agar + 12 grams HiVeg LB broth + 600 mL ddH2O<br><br> | |
| - | Made LB broth using 10 grams of bacto agar + 12 grams HiVeg LB broth + 600 mL ddH2O<br> | + | Autoclaved 1.5 mL Eppendorf tubes as well as LB<br><br> |
| - | Autoclaved 1.5 mL Eppendorf tubes as well as LB<br> | + | Poured plates of LB and chloramphenicol (CAM)<br><br> |
| - | Poured plates of LB and chloramphenicol (CAM)<br> | + | Streaked 2 plates with E. coli DguaB (pDCAF3)<br><br> |
| - | Streaked 2 plates with E. coli DguaB (pDCAF3)<br> | + | Incubated in 37 degrees celsius<br><br> |
| - | Incubated in 37 degrees celsius<br> | + | Aliquoted kanamycin (KAN) in four 1mL tubes<br><br> |
| - | Aliquoted kanamycin (KAN) in four 1mL tubes<br> | + | |
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| - | <h2> | + | <h2>Tuesday 10<sup>th</sup> June</h2> |
| - | <a href="https:// | + | <a href="https://2014.igem.org/Team:UIUC_Illinois/Project/Overview" target="_blank" class="tbnotelogo PSlogo"> ASDF </a> |
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| - | Autoclaved at 1:30pm<br> | + | Autoclaved at 1:30pm<br><br> |
<ul style="text-indent:30px;"> | <ul style="text-indent:30px;"> | ||
<li>0.8 grams of YNB + 156 mL H2O</li> | <li>0.8 grams of YNB + 156 mL H2O</li> | ||
<li>28 grams of salts + 490 mL H2O</li> | <li>28 grams of salts + 490 mL H2O</li> | ||
</ul><br> | </ul><br> | ||
| - | Used sterile filter for 1M MgSO4 and 1M CaCl2<br> | + | Used sterile filter for 1M MgSO4 and 1M CaCl2<br><br> |
| - | Suspended 5 uL of CM34 (antibiotic) + 5 mL of LB + 1 colony of pDCAF3 E. coli<br> | + | Suspended 5 uL of CM34 (antibiotic) + 5 mL of LB + 1 colony of pDCAF3 E. coli<br><br> |
| - | Added .5 grams of caffeine + 400 uL of 1M MgSO4 + 20 uL 1M CaCl2 + 40 mL M95x salts + H2O to YNB + H2O mixture<br> | + | Added .5 grams of caffeine + 400 uL of 1M MgSO4 + 20 uL 1M CaCl2 + 40 mL M95x salts + H2O to YNB + H2O mixture<br><br> |
| - | <ul><li>Labeled this "M9 media"</li></ul><br> | + | <ul style="text-index:30px;"><li>Labeled this "M9 media"</li></ul><br> |
| - | Inoculated frozen stock of E. coli guaB pDCAF3 in 5 mL of M9 media<br> | + | Inoculated frozen stock of E. coli guaB pDCAF3 in 5 mL of M9 media<br><br> |
| - | Placed sample in 30 degree celsius shaker for culturing<br> | + | Placed sample in 30 degree celsius shaker for culturing<br><br> |
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Revision as of 01:39, 17 October 2014
-
PROJECT NOTES
- NOTES BY MONTH
Monday 9th June
ASDF Made LB broth using 10 grams of bacto agar + 12 grams HiVeg LB broth + 600 mL ddH2OAutoclaved 1.5 mL Eppendorf tubes as well as LB
Poured plates of LB and chloramphenicol (CAM)
Streaked 2 plates with E. coli DguaB (pDCAF3)
Incubated in 37 degrees celsius
Aliquoted kanamycin (KAN) in four 1mL tubes
Tuesday 10th June
ASDF Autoclaved at 1:30pm- 0.8 grams of YNB + 156 mL H2O
- 28 grams of salts + 490 mL H2O
Used sterile filter for 1M MgSO4 and 1M CaCl2
Suspended 5 uL of CM34 (antibiotic) + 5 mL of LB + 1 colony of pDCAF3 E. coli
Added .5 grams of caffeine + 400 uL of 1M MgSO4 + 20 uL 1M CaCl2 + 40 mL M95x salts + H2O to YNB + H2O mixture
- Labeled this "M9 media"
Inoculated frozen stock of E. coli guaB pDCAF3 in 5 mL of M9 media
Placed sample in 30 degree celsius shaker for culturing
The Institute for Genomic Biology
Biosystems Design, South wing, 2nd floor
University of Illinois at Urbana-Champaign
1206 West Gregory Drive
61801 Urbana, Illinois
illinoisigem@gmail.com
illinoisigem@gmail.com 
