Team:Paris Saclay/Notebook/July/8

From 2014.igem.org

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''E. coli'' strain used:
''E. coli'' strain used:
* XL1-Blue
* XL1-Blue
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[https://2013.igem.org/Team:Paris_Saclay/extraction Protocol]

Revision as of 20:19, 9 July 2014

Contents

Tuesday 8th July

Lab work

1 - Rehydration of the following biobricks:

Sean

  • BBa B0015(CmR)
  • BBa J23119(CmR)
  • BBa K731020(CmR)
  • BBa K206000(CmR)
  • BBa J04500(CmR)
  • BBa J23100(AmpR)
  • BBa J23106(AmpR)
  • BBa J23114(AmpR)


Protocol:

  1. Pierce foil cover of well containing biobrick of interest.
  2. Add 10 μL of water in well.
  3. Transfer contents of well into a 1.5 mL microcentrifuge tube.

2 - Transformation in DH5α of the following biobricks:

Mathieu & Maher

  • BBa B0015(CmR)
  • BBa J23119(CmR)
  • BBa K731020(CmR)
  • BBa K206000(CmR)
  • BBa J04500(CmR)
  • BBa J23100(AmpR)
  • BBa J23106(AmpR)
  • BBa J23114(AmpR
  • Control + : pUC18 (20ng)
  • Control -


Protocol

NB : The protocol was modified. Instead of spreading 10-1 and 10-2 solutions of bacteria we took a non diluted solution and a 10-1 solution.

3 - Plasmid DNA preparation:

Romain & Sean

Plasmids used:

  • pJBEI-6409


E. coli strain used:

  • XL1-Blue

Protocol

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