Team:UIUC Illinois/Project/PuppyProbiotic

From 2014.igem.org

(Difference between revisions)
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<br>Canines and many small animals are incapable of metabolizing theobromine and related stimulants as fast as humans can. Therefore, an excess of the stimulant builds up and begins to overstimulate the animal, thus leading to many malicious side effects. Ranging from slight nausea to death, consumption of these compounds are harmful for the animal. In fact, theobromine has been employed to ward off pests of the same genus as canines: "Results indicate that mixtures of theobromine and caffeine have the potential to be developed into a selective, effective, and socially acceptable toxicant for the control of pest coyotes."[1]
<br>Canines and many small animals are incapable of metabolizing theobromine and related stimulants as fast as humans can. Therefore, an excess of the stimulant builds up and begins to overstimulate the animal, thus leading to many malicious side effects. Ranging from slight nausea to death, consumption of these compounds are harmful for the animal. In fact, theobromine has been employed to ward off pests of the same genus as canines: "Results indicate that mixtures of theobromine and caffeine have the potential to be developed into a selective, effective, and socially acceptable toxicant for the control of pest coyotes."[1]
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  <script type="text/javascript" src="script.js.pagespeed.jm.0cDO9dEC4I.js"></script>
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  <div class="chocolate-meter-bg rounded-corners20 clearfix">
 +
  <!-- Results Section -->
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  <div class="Results">
 +
    <div id="welcome">
 +
      <span class="red">The petMD Chocolate Toxicity Meter will let you know how much theobromine and caffeine your dog has consumed and the symptoms that may follow.</span>
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 +
<span>Enter your dog's weight, the type of chocolate, and the amount consumed to get started.</span>
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    </div>
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    <div id="running">
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    <div id="status" class="status bold"></div>
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    <div id="advice" class="advice mb15 bold"></div>
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    <div class="symptoms Arial">
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        <div id="secondary"></div>
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        <div id="concerns"></div> 
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        <div id="description" class="description mb10 bold">If you notice any of these symptoms contact the Pet Poison Helpline at 1-855-213-6680 or your veterinarian right away.</div>
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        <div class="mb15 center"><img src="data:image/webp;base64,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" alt="Pet Poison Hotline"></div>
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  </div>
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</div>
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</div>
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<!-- End -->
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<div class="light-brown-outside rounded-corners20">
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  <div class="brown-inside-small rounded-corners20">
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    <div class="white-box-small rounded-corners15">
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      <div class="bar-wrapper rounded-corners15">
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        <div id="bar" class="progress">
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          &nbsp;
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        </div>
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      </div>
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      <div class="under_bar">
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      <div id="statusMain" class="insta_results status">Welcome!</div>
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      </div> 
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  </div>
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</div>
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<span class="dog-n-bones"><img src="data:image/webp;base64,UklGRmgBAABXRUJQVlA4IFwBAAAwCgCdASpHACEAPoUykkenpCIhtVs7MPAQiWIAzbKzRIDbjFXAKka9ezYuvEJJdm7mItmol0Qe6altGgHmk7v9VQZGsxVq5xHFNOJJJ0c/NEDh7x5qvWwI4AAA/vstopBkYdNnf+ftUDWgHVh8MKy/F6r7RGNvjLvk+7XkEPo4goNxrVJKgviwtmzzPyn9b9CmXdQeV6W4cScybK2Z1i+/RQ1fxkVABfImeC5wengr2+O5XkeEq5M2LFCMy/KABgmb8QvtZEX6if3JD9tFWq6GBLH9DTZwzZhmnw4sNWPMi1GI4HoDN8Q8na/XKfqAHZ8S5rm2rYU9uDnTE5LU20Eu1DKosuKCnENsg1qMs5wpqbqJsB5YKlNkcQBGpR1rb0LsViMsN9etJZojnfNIsITN/iE0pqrPEeyD+06Jzf+loKMjBz1jRpfuEe4BnwAdjm/eQoKF7GMmwEdAAAA="></span>
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<div class="brown-inside-big rounded-corners20">
 +
  <div class="white-box-big rounded-corners15">
 +
      <div class="Mid_Wrapper">
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          <div class="mb10 clearfix">
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              <div class="label">Dog's Weight</div>
 +
              <div class="floatR">
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              <div class="input">
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                  <input type="text" name="weight" id="weight" value="10">
 +
                  <div class="controls">
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                    <span class="up"></span>
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                    <span class="down"></span>
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                  </div>
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              </div>
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                <span class="label tag">lbs.</span>
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          </div>
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        </div>
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          <div class="lightBot">
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              <div class="slider">
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                  <div id="weightSlide" class="noUiSlider"></div>
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              </div>
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          </div>
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          <div class="darkTop mb10 tp25 clearfix">
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              <div class="label select">Chocolate Type</div>
 +
              <div class="floatR">
 +
                <select name="chock" id="chock">
 +
                    <option value="milk">Milk Chocolate</option>
 +
                    <option value="dark">Dark Chocolate</option>
 +
                    <option value="bakers">Bakers Chocolate</option>
 +
                    <option value="semi">Semi-Sweet Chocolate </option>
 +
                    <option value="cocoa">Cocoa Powder</option>
 +
                    <option value="white">White Chocolate</option>
 +
                </select>
 +
              </div>
 +
          </div>
 +
          <div class="mb15 clearfix">
 +
              <div class="label">Amount Consumed</div>
 +
           
 +
              <div class="floatR">
 +
                <div class="input">
 +
                    <input type="text" name="oz" id="oz" value="0.0">
 +
                    <div class="controls">
 +
                      <span class="up"></span>
 +
                      <span class="down"></span>
 +
                    </div>
 +
                </div>
 +
                <span class="label tag">oz.</span>
 +
              </div>
 +
          </div>
 +
      </div>
 +
      <div class="measurements"></div>
 +
    </div>
 +
  </div>
 +
</div>
 +
<div class="learnMore">Read more about Chocolate Poisoning in Dogs <a target="_parent" href="/dog/conditions/digestive/c_multi_chocolate_toxicity">Here</a></div>
 +
</div>
 +
  <script type="text/javascript">$(document).ready(function(){$('#weightSlide').noUiSlider('init',{handles:1,connect:"lower",scale:[10,210],start:10,change:function(){var current=$('#weightSlide').noUiSlider('value');$('#weight').val(current[1]);chocoMeter.refreshMeter();},});chocoMeter.moveBar(0);$('input').bind({keyup:function(){chocoMeter.refreshMeter();},keydown:function(){if(!chocoMeter.onlyNumbers(event))
 +
event.preventDefault();}});$('#weight').change(function(event){chocoMeter.moveSlider($(this).val());});$('.up').click(chocoMeter.increase);$('.down').click(chocoMeter.decrease);$("#chock").selectBox().change(function(){chocoMeter.refreshMeter();});});</script>
 +
</body>
 +
</html>
</br>
</br>
   </div>
   </div>

Revision as of 01:34, 17 October 2014


Puppy Probiotics.

An Introduction


Imagine...
Imagine drinking a cup of coffee. You stolidly sip your espresso and soon enough you feel a warm sensation cajole your body into getting that assignment done. However, you notice you're fidgeting uncontrollably, the room starts spinning and you begin to vomit. Ambulances, the noises are familiar, you've just seized. You're a dog. Smiley face

What just happened?
Canines and many small animals are incapable of metabolizing theobromine and related stimulants as fast as humans can. Therefore, an excess of the stimulant builds up and begins to overstimulate the animal, thus leading to many malicious side effects. Ranging from slight nausea to death, consumption of these compounds are harmful for the animal. In fact, theobromine has been employed to ward off pests of the same genus as canines: "Results indicate that mixtures of theobromine and caffeine have the potential to be developed into a selective, effective, and socially acceptable toxicant for the control of pest coyotes."[1]

The petMD Chocolate Toxicity Meter will let you know how much theobromine and caffeine your dog has consumed and the symptoms that may follow. Enter your dog's weight, the type of chocolate, and the amount consumed to get started.
If you notice any of these symptoms contact the Pet Poison Helpline at 1-855-213-6680 or your veterinarian right away.
Pet Poison Hotline
 
Welcome!
Dog's Weight
lbs.
Chocolate Type
Amount Consumed
oz.
Read more about Chocolate Poisoning in Dogs Here
</br> 

 </div>

Training the Team Underwent

Have your team members received any safety training yet? Our team had two types of training. The first was an online training by the DRS or the Division of Research Safety. This safety training included multiple quizzes and tutorials for General Laboratory Safety and Understanding BioSafety. Additionally, our team took a synthetic biology bootcamp at the beginning of the year to cover general laboratory procedures and safety guidelines. Other safety training such as autoclaving came at another time.

Please briefly describe the topics that you learned about (or will learn about) in your safety training. For all techniques listed, our lab required usage of gloves, goggles, lab coats, closed-toe shoes and long pants to minimize the amount of contact between us and the materials that are being used. Also, general aseptic protocols were adhered to when handling any lab techniques to prevent contamination. Our lab required us to go through a live training with a lab representative to learn proper handling and care of the autoclave.

Who is responsible for biological safety at your institution? BioSafety is all handled by the Division of Research Safety or DRS. As long as it is BioSafety Level 1, our lab, The Institute of Genomic Biology, has no concerns and just requires us to take training through the DRS. The training for the DRS includes online presentations to teach us safety techniques. These presentations are followed by online quizzes that test what we have learn to earn certification. The certification enables us usage of the lab.

</html>


The Institute for Genomic Biology
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University of Illinois at Urbana-Champaign
1206 West Gregory Drive
61801 Urbana, Illinois
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Copyright (c) 2014 igem.org. All rights reserved.
Retrieved from "http://2014.igem.org/Team:UIUC_Illinois/Project/PuppyProbiotic"