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Team:UIUC Illinois/Notebook
From 2014.igem.org
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<a href="https://2013.igem.org/Team:Paris_Bettencourt/Project/Detect" target="_blank" class="tbnotelogo PSlogo"> ASDF </a> | <a href="https://2013.igem.org/Team:Paris_Bettencourt/Project/Detect" target="_blank" class="tbnotelogo PSlogo"> ASDF </a> | ||
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| - | <h3> | + | <h3>Monday 9<sup>th</sup> June</h3> |
Made LB broth using 10 grams of bacto agar + 12 grams HiVeg LB broth + 600 mL ddH2O<br> | Made LB broth using 10 grams of bacto agar + 12 grams HiVeg LB broth + 600 mL ddH2O<br> | ||
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| + | <h2>Title</h2> | ||
| + | <a href="https://2013.igem.org/Team:Paris_Bettencourt/Project/Detect" target="_blank" class="tbnotelogo PSlogo"> ASDF </a> | ||
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| + | <h3>Tuesday 10<sup>th</sup> June</h3> | ||
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| + | Autoclaved at 1:30pm<br> | ||
| + | <ul> | ||
| + | <li>0.8 grams of YNB + 156 mL H2O</li> | ||
| + | <li>28 grams of salts + 490 mL H2O</li> | ||
| + | </ul> | ||
| + | Used sterile filter for 1M MgSO4 and 1M CaCl2<br> | ||
| + | Suspended 5 uL of CM34 (antibiotic) + 5 mL of LB + 1 colony of pDCAF3 E. coli<br> | ||
| + | Added .5 grams of caffeine + 400 uL of 1M MgSO4 + 20 uL 1M CaCl2 + 40 mL M95x salts + H2O to YNB + H2O mixture<br> | ||
| + | <ul><li>Labeled this "M9 media"</li></ul> | ||
| + | Inoculated frozen stock of E. coli guaB pDCAF3 in 5 mL of M9 media<br> | ||
| + | Placed sample in 30 degree celsius shaker for culturing<br> | ||
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Revision as of 01:17, 17 October 2014
-
DAY NOTES
- NOTES
Title
ASDFMonday 9th June
Made LB broth using 10 grams of bacto agar + 12 grams HiVeg LB broth + 600 mL ddH2OAutoclaved 1.5 mL Eppendorf tubes as well as LB
Poured plates of LB and chloramphenicol (CAM)
Streaked 2 plates with E. coli DguaB (pDCAF3)
Incubated in 37 degrees celsius
Aliquoted kanamycin (KAN) in four 1mL tubes
Title
ASDFTuesday 10th June
Autoclaved at 1:30pm- 0.8 grams of YNB + 156 mL H2O
- 28 grams of salts + 490 mL H2O
Suspended 5 uL of CM34 (antibiotic) + 5 mL of LB + 1 colony of pDCAF3 E. coli
Added .5 grams of caffeine + 400 uL of 1M MgSO4 + 20 uL 1M CaCl2 + 40 mL M95x salts + H2O to YNB + H2O mixture
- Labeled this "M9 media"
Placed sample in 30 degree celsius shaker for culturing
The Institute for Genomic Biology
Biosystems Design, South wing, 2nd floor
University of Illinois at Urbana-Champaign
1206 West Gregory Drive
61801 Urbana, Illinois
illinoisigem@gmail.com
illinoisigem@gmail.com 
