Team:Austin Texas/kit
From 2014.igem.org
(→Experimental Preparation) |
|||
Line 156: | Line 156: | ||
If the cultures did not have IPTG or an nCAA, an equal volume of sterile deionized water was added in order to keep the volumes between cultures constant. Once the water, the IPTG, and the ncAA were added appropriately, the cultures were allowed to grow to ~0.5 OD 600. 70 µL of each culture condition and control culture were added to a separate well in a transparent 96-well plate for fluorescence and OD readings using a fluorometer. | If the cultures did not have IPTG or an nCAA, an equal volume of sterile deionized water was added in order to keep the volumes between cultures constant. Once the water, the IPTG, and the ncAA were added appropriately, the cultures were allowed to grow to ~0.5 OD 600. 70 µL of each culture condition and control culture were added to a separate well in a transparent 96-well plate for fluorescence and OD readings using a fluorometer. | ||
- | |||
- | |||
Line 163: | Line 161: | ||
[[File:10-14-14 big experiment test tubes.png|450px|thumb|left| Preparation for the ncAA Kit Test]] | [[File:10-14-14 big experiment test tubes.png|450px|thumb|left| Preparation for the ncAA Kit Test]] | ||
[[File:RFP-GFP Controls 10-14-15.png|200px|thumb|right| A picture showing culture with or without IPTG. Without IPTG, there is no induction of GFP (left). With IPTG, strong fluorescence is easily seen (right).]] | [[File:RFP-GFP Controls 10-14-15.png|200px|thumb|right| A picture showing culture with or without IPTG. Without IPTG, there is no induction of GFP (left). With IPTG, strong fluorescence is easily seen (right).]] | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
=Results and Data= | =Results and Data= |
Revision as of 01:12, 17 October 2014
|