"
Team:Cambridge-JIC/Guide/Materials/Equipment
From 2014.igem.org
(Difference between revisions)
(Created page with "- Marchantia Growth You will need a room/compartment at room temperature under full illumination. Ventilation is also recommended, but not essential Transformation For transform...") |
|||
| Line 1: | Line 1: | ||
| - | + | =Physical Equipment= | |
| - | Growth | + | ==Marchantia== |
| + | ===Growth=== | ||
You will need a room/compartment at room temperature under full illumination. Ventilation is also recommended, but not essential | You will need a room/compartment at room temperature under full illumination. Ventilation is also recommended, but not essential | ||
| - | Transformation | + | ===Transformation=== |
For transformation using [[cocultivation]], you will need a shaker under full illumination capable of shaking 200ml conical flasks. | For transformation using [[cocultivation]], you will need a shaker under full illumination capable of shaking 200ml conical flasks. | ||
Otherwise, a ventilated illumination space at room temperature is sufficient. | Otherwise, a ventilated illumination space at room temperature is sufficient. | ||
| - | Other | + | ===Other=== |
Porous tape to seal plates from fungal contamination, but enabling gas exchange, is essential | Porous tape to seal plates from fungal contamination, but enabling gas exchange, is essential | ||
| - | + | ==Agrobacteria== | |
| - | Growth | + | ===Growth=== |
You will need a 30degree incubator, as well as a room temperature shaker for growing liquid cultures. | You will need a 30degree incubator, as well as a room temperature shaker for growing liquid cultures. | ||
| - | Transformation | + | ===Transformation=== |
| - | You will need electroporation equipment capable of delivering at least 1.25kV (if using 1mm cuvettes) | + | You will need electroporation equipment capable of delivering at least 1.25kV (if using 1mm cuvettes). Do not build your own unless you have the prerequisite safety qualifications! |
Revision as of 00:41, 17 October 2014
Contents |
Physical Equipment
Marchantia
Growth
You will need a room/compartment at room temperature under full illumination. Ventilation is also recommended, but not essential
Transformation
For transformation using cocultivation, you will need a shaker under full illumination capable of shaking 200ml conical flasks.
Otherwise, a ventilated illumination space at room temperature is sufficient.
Other
Porous tape to seal plates from fungal contamination, but enabling gas exchange, is essential
Agrobacteria
Growth
You will need a 30degree incubator, as well as a room temperature shaker for growing liquid cultures.
Transformation
You will need electroporation equipment capable of delivering at least 1.25kV (if using 1mm cuvettes). Do not build your own unless you have the prerequisite safety qualifications!
