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	==Links==		==Links==
-	The rough protocol is:	+	The steps in transformation are:
-	1. Electroporate agrobacterium	+
-	2. Prepare spores	+	1. [[Team:Cambridge-JIC/Guide/Transformation/TransformingAgro | Electroporate Agrobacterium]]
-	3. Cocultivate or use AgarTrap	+
-	4. Plate transformants	+	2. [[Team:Cambridge-JIC/Guide/Care/PreparingSpores | Prepare spores]]
		+
		+	3. [[Team:Cambridge-JIC/Guide/Transformation/Cocultivation | Cocultivate]] or use [[Team:Cambridge-JIC/Guide/Transformation/AgarTrap| AgarTrap]]
		+
		+	4. [[Team:Cambridge-JIC/Guide/Transformation/SelectingTransformants| Select transformants]]

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Revision as of 00:23, 17 October 2014

Transformation

This section contains the information for transforming Marchantia spores. The best established procedures take around 7 days.

See the Team:Cambridge-JIC/Guide/Transformation/Outline outline for an overview of the process.

Links

The steps in transformation are:

1. Electroporate Agrobacterium

2. Prepare spores

3. Cocultivate or use AgarTrap

4. Select transformants