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Team:Cambridge-JIC/Guide/Transformation/SelectingTransformants
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If using the [http://parts.igem.org/Part:BBa_K1484316 Marchantia selection cassette]], plate spores onto [[Team:Cambridge-JIC/Guide/Materials/Media | 1/2 B5 + 100ug/ml cefotaxime + 10ug/ml hygromycin + 1.2% agar plates]], and leave under full illumination at room temperature to grow. | If using the [http://parts.igem.org/Part:BBa_K1484316 Marchantia selection cassette]], plate spores onto [[Team:Cambridge-JIC/Guide/Materials/Media | 1/2 B5 + 100ug/ml cefotaxime + 10ug/ml hygromycin + 1.2% agar plates]], and leave under full illumination at room temperature to grow. | ||
| - | Always do a negative control for the transformation to grow. Within 3-5 days, the difference | + | Always do a negative control for the transformation to grow. Within 3-5 days, the difference between the transformation plate and the control plate is apparent. Fluorescent transformants appear from 3 days onwards. |
Plates often dry out within 2-3 weeks. You will need to [[Team:Cambridge-JIC/Guide/Care/Repropagating| repropagate]] the transformed plantlets to get growth of isogenic gemmae. | Plates often dry out within 2-3 weeks. You will need to [[Team:Cambridge-JIC/Guide/Care/Repropagating| repropagate]] the transformed plantlets to get growth of isogenic gemmae. | ||
Revision as of 00:14, 17 October 2014
Selecting Transformants
If using the [http://parts.igem.org/Part:BBa_K1484316 Marchantia selection cassette]], plate spores onto 1/2 B5 + 100ug/ml cefotaxime + 10ug/ml hygromycin + 1.2% agar plates, and leave under full illumination at room temperature to grow.
Always do a negative control for the transformation to grow. Within 3-5 days, the difference between the transformation plate and the control plate is apparent. Fluorescent transformants appear from 3 days onwards.
Plates often dry out within 2-3 weeks. You will need to repropagate the transformed plantlets to get growth of isogenic gemmae.
