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Team:Cambridge-JIC/Guide/Transformation/SelectingTransformants
From 2014.igem.org
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| + | If using the [http://parts.igem.org/Part:BBa_K1484316 Marchantia selection cassette]], plate spores onto [[Team:Cambridge-JIC/Guide/Materials/Media | 1/2 B5 + 100ug/ml cefotaxime + 10ug/ml hygromycin + 1.2% agar plates]], and leave under full illumination at room temperature to grow. | ||
Always do a negative control for the transformation to grow. Within 3-5 days, the difference from the control plate is apparent. Fluorescent transformants appear from 3 days onwards. | Always do a negative control for the transformation to grow. Within 3-5 days, the difference from the control plate is apparent. Fluorescent transformants appear from 3 days onwards. | ||
Revision as of 00:11, 17 October 2014
Selecting Transformants
If using the [http://parts.igem.org/Part:BBa_K1484316 Marchantia selection cassette]], plate spores onto 1/2 B5 + 100ug/ml cefotaxime + 10ug/ml hygromycin + 1.2% agar plates, and leave under full illumination at room temperature to grow.
Always do a negative control for the transformation to grow. Within 3-5 days, the difference from the control plate is apparent. Fluorescent transformants appear from 3 days onwards.
