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Team:Groningen/Template/MODULE/Notebook/toolbox/appendix/table3
From 2014.igem.org
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Revision as of 00:04, 17 October 2014
Primers for making BioBricks out of genes with illegal restriction sites
| Gene | Primer | Sequence |
|---|---|---|
| NisT | Prefix forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGATGAAGTGAAAGAATTTACATCA |
| S980 reverse | TTGTTCCATAAACAAACTTGTATTATAAATGATGTAAATA | |
| S980 forward | TATTTACATCATTTATAATACAAGTTTGTTTATGGAACAA | |
| S1210 reverse | CCATAGTTGGTTGATATAATCCTGAAATTATCTTTACTTGTGTAC | |
| S1210 forward | GTACACAAGTAAAGATAATTTCAGGATTATATCAACCAACTATGG | |
| Suffix reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTCATCATTATCCT | |
| Suffix forward | ATGAATAATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA | |
| Prefix reverse | CACTTCATCCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG | |
| NisP | Prefix forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGGTGAAAAAAATACTAGGTTT |
| P225 reverse | AGTCCTTGTTGTCGATTTTACTGCTGGTGACTGCGCCCCT | |
| P225 forward | AGGGGCGCAGTCACCAGCAGTAAAATCGACAACAAGGACT | |
| E347 reverse | AACGCTATCTCTCTTACTAAATTCAGAACTAACTTGAGTT | |
| E347 forward | AACTCAAGTTAGTTCTGAATTTAGTAAGAGAGATAGCGTT | |
| E1657 reverse | CAATACTCTATTCCCATTAACTTCTGGACTATTCATTAGA | |
| E1657 forward | TCTAATGAATAGTCCAGAAGTTAATGGGAATAGAGTATTG | |
| Suffix reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCAATTTTTAGTCTTTC | |
| Suffix forward | AAATTGATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA | |
| Prefix reverse | CCTAGTATTTTTTTCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG | |
| NisFEG | Prefix forward | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAGGTAAAAATTCAAAA |
| P186 reverse | TCCAGTATCAGCAGAAATTAAACCAAACAAAATTTTCATC | |
| P186 forward | GATGAAAATTTTGTTTGGTTTAATTTCTGCTGATACTGGA | |
| E336/X349 reverse | CCTGTTTCTGCCAAACCAATCACTTCTAGTGTTTCATGTATTCTCTTA | |
| E336/X349 forward | TAAGAGAATACATGAAACACTAGAAGTGATTGGTTTGGCAGAAACAGG | |
| Suffix reverse | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATCTAATCTTTTTTT | |
| Suffix forward | TAGATAATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA | |
| Prefix reverse | TTTTACCTGCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG |
