Team:Groningen/Template/MODULE/Notebook/toolbox/appendix/table3

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Primers for making BioBricks out of genes with illegal restriction sites
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<table class="table">
<table class="table">
-
<tr>
+
<tr>  
-
<th>type</th><th>size (mm)</th>
+
    <th>Gene</th>  
-
</tr>
+
    <th>Primer</th>
-
<tr>
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    <th>Sequence</th>
-
<td>colony</td><td>4,0</td>
+
  </tr>
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</tr>
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  <tr>
-
<tr>
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    <td rowspan="8">NisT</td>
-
<td>colony with halo</td><td>6,0</td>
+
    <td>Prefix forward</td>
-
</tr>
+
    <td>GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGATGAAGTGAAAGAATTTACATCA</td>
-
<tr>
+
  </tr>
-
<td>halo</td><td>2,0</td>
+
  <tr>
-
</tr>
+
    <td>S980 reverse</td>
-
 
+
    <td>TTGTTCCATAAACAAACTTGTATTATAAATGATGTAAATA</td>
 +
  </tr>
 +
  <tr>
 +
    <td>S980 forward</td>
 +
    <td>TATTTACATCATTTATAATACAAGTTTGTTTATGGAACAA</td>
 +
  </tr>
 +
  <tr>
 +
    <td>S1210 reverse</td>
 +
    <td>CCATAGTTGGTTGATATAATCCTGAAATTATCTTTACTTGTGTAC</td>
 +
  </tr>
 +
  <tr>
 +
    <td>S1210 forward</td>
 +
    <td>GTACACAAGTAAAGATAATTTCAGGATTATATCAACCAACTATGG</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Suffix reverse</td>
 +
    <td>GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTCATCATTATCCT</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Suffix forward</td>
 +
    <td>ATGAATAATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Prefix reverse</td>
 +
    <td>CACTTCATCCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG</td>
 +
  </tr> 
 +
  <tr>
 +
    <td rowspan="10">NisP</td>
 +
    <td>Prefix forward</td>
 +
    <td>GTTTCTTCGAATTCGCGGCCGCTTCTAGGTGAAAAAAATACTAGGTTT</td>
 +
  <tr>
 +
    <td>P225 reverse</td>
 +
    <td>AGTCCTTGTTGTCGATTTTACTGCTGGTGACTGCGCCCCT</td>
 +
  </tr>
 +
  <tr>
 +
    <td>P225 forward</td>
 +
    <td>AGGGGCGCAGTCACCAGCAGTAAAATCGACAACAAGGACT</td>
 +
  </tr>
 +
  <tr>
 +
    <td>E347 reverse</td>
 +
    <td>AACGCTATCTCTCTTACTAAATTCAGAACTAACTTGAGTT</td>
 +
  </tr>
 +
  <tr>
 +
    <td>E347 forward</td>
 +
    <td>AACTCAAGTTAGTTCTGAATTTAGTAAGAGAGATAGCGTT</td>
 +
  </tr>
 +
  <tr>
 +
    <td>E1657 reverse</td>
 +
    <td>CAATACTCTATTCCCATTAACTTCTGGACTATTCATTAGA</td>
 +
  </tr>
 +
  <tr>
 +
    <td>E1657 forward</td>
 +
    <td>TCTAATGAATAGTCCAGAAGTTAATGGGAATAGAGTATTG</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Suffix reverse</td>
 +
    <td>GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCAATTTTTAGTCTTTC</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Suffix forward</td>
 +
    <td>AAATTGATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Prefix reverse</td>
 +
    <td>CCTAGTATTTTTTTCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG</td>
 +
  </tr>
 +
  <tr>
 +
    <td rowspan="8">NisFEG</td>
 +
    <td>Prefix forward</td>
 +
    <td>GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAGGTAAAAATTCAAAA</td>
 +
  </tr>
 +
  <tr>
 +
    <td>P186 reverse</td>
 +
    <td>TCCAGTATCAGCAGAAATTAAACCAAACAAAATTTTCATC</td>
 +
  </tr>
 +
  <tr>
 +
    <td>P186 forward</td>
 +
    <td>GATGAAAATTTTGTTTGGTTTAATTTCTGCTGATACTGGA</td>
 +
  </tr>
 +
  <tr>
 +
    <td>E336/X349 reverse</td>
 +
    <td>CCTGTTTCTGCCAAACCAATCACTTCTAGTGTTTCATGTATTCTCTTA</td>
 +
  </tr>
 +
  <tr>
 +
    <td>E336/X349 forward</td>
 +
    <td>TAAGAGAATACATGAAACACTAGAAGTGATTGGTTTGGCAGAAACAGG</td>
 +
  </tr>
 +
      <tr>
 +
    <td>Suffix reverse</td>
 +
    <td>GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATCTAATCTTTTTTT</td>
 +
  </tr>
 +
  </tr>
 +
      <tr>
 +
    <td>Suffix forward</td>
 +
    <td>TAGATAATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA</td>
 +
  </tr> 
 +
  <tr>
 +
    <td>Prefix reverse</td>
 +
    <td>TTTTACCTGCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG</td>
 +
  </tr>   
</table>
</table>
<div class="hspacer">&nbsp;</div>
<div class="hspacer">&nbsp;</div>

Revision as of 00:02, 17 October 2014

Primers for making BioBricks out of genes with illegal restriction sites
 
Gene Primer Sequence
NisT Prefix forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGATGAAGTGAAAGAATTTACATCA
S980 reverse TTGTTCCATAAACAAACTTGTATTATAAATGATGTAAATA
S980 forward TATTTACATCATTTATAATACAAGTTTGTTTATGGAACAA
S1210 reverse CCATAGTTGGTTGATATAATCCTGAAATTATCTTTACTTGTGTAC
S1210 forward GTACACAAGTAAAGATAATTTCAGGATTATATCAACCAACTATGG
Suffix reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTCATCATTATCCT
Suffix forward ATGAATAATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA
Prefix reverse CACTTCATCCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG
NisP Prefix forward GTTTCTTCGAATTCGCGGCCGCTTCTAGGTGAAAAAAATACTAGGTTT
P225 reverse AGTCCTTGTTGTCGATTTTACTGCTGGTGACTGCGCCCCT
P225 forward AGGGGCGCAGTCACCAGCAGTAAAATCGACAACAAGGACT
E347 reverse AACGCTATCTCTCTTACTAAATTCAGAACTAACTTGAGTT
E347 forward AACTCAAGTTAGTTCTGAATTTAGTAAGAGAGATAGCGTT
E1657 reverse CAATACTCTATTCCCATTAACTTCTGGACTATTCATTAGA
E1657 forward TCTAATGAATAGTCCAGAAGTTAATGGGAATAGAGTATTG
Suffix reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCAATTTTTAGTCTTTC
Suffix forward AAATTGATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA
Prefix reverse CCTAGTATTTTTTTCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG
NisFEG Prefix forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAGGTAAAAATTCAAAA
P186 reverse TCCAGTATCAGCAGAAATTAAACCAAACAAAATTTTCATC
P186 forward GATGAAAATTTTGTTTGGTTTAATTTCTGCTGATACTGGA
E336/X349 reverse CCTGTTTCTGCCAAACCAATCACTTCTAGTGTTTCATGTATTCTCTTA
E336/X349 forward TAAGAGAATACATGAAACACTAGAAGTGATTGGTTTGGCAGAAACAGG
Suffix reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATCTAATCTTTTTTT
Suffix forward TAGATAATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA
Prefix reverse TTTTACCTGCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG
 
 
 
 
 

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