Team:Austin Texas/kit

From 2014.igem.org

(Difference between revisions)
(Background)
(Incorporation Value)
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'''Shouldn't we use "the highest incorporation values..."?'''
'''Shouldn't we use "the highest incorporation values..."?'''
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The synthetase/tRNA pairs that showed the highest level of ncAA incorporation include 3-iodotyrosine, 4-azidophenylalanine, 3-nitrotyrosine, and ortho-nitrobenzyltyrosine in decreasing order '''Is this order correct?'''. Among the ncAAs tested, ONBY consistently slowed the growth rate of the culture significantly  (as seen by OD600 readings, data not shown) suggesting possible toxicity to the cell.  However, ncAA incorporation remained high.  Synthetase/tRNA pairs that showed the lowest levels of ncAA incorporation include 3-aminotyrosine, <small>L</small>-DOPA, tyrosine, and 4-cyanophenylalanine.
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The synthetase/tRNA pairs that showed the highest level of ncAA incorporation include 4-azidophenylalanine, 3-nitrotyrosine, ortho-nitrobenzyltyrosine, and 3-iodotyrosine in decreasing order. Among the ncAAs tested, ONBY consistently slowed the growth rate of the culture significantly  (as seen by OD600 readings, data not shown) suggesting possible toxicity to the cell.  However, ncAA incorporation remained high.  Synthetase/tRNA pairs that showed the lowest levels of ncAA incorporation include 3-aminotyrosine, <small>L</small>-DOPA, and 4-cyanophenylalanine.
=Discussion=
=Discussion=

Revision as of 23:26, 16 October 2014