Team:LZU-China

From 2014.igem.org

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<head>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=gb2312" />
<meta http-equiv="Content-Type" content="text/html; charset=gb2312" />
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<title>iGEM LZU-CHINA 2014</title>
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<title>LZU-China 2014</title>
<style type="text/css">
<style type="text/css">
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*{
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font-size:14pt;  /*ZH*/
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font-family:微软雅黑; /**/
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width:105px; /**/
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#nav{
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padding:0;} #nav li{ /**/
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float:left; /**/
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text-align:center;
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}
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#nav li a{
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color:rgba(190,194,203,0); /**/
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background:rgba(190,194,203,0); /**/
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height:61px;/*高度*/
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}
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#nav li a:hover{
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color:rgba(190,194,203,0); /**/
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background:rgba(190,194,203,0); /**/
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font-family: "微软雅黑";
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#nav li ul{
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margin:0px;/**/
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#nav li ul li{
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float:none;
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}
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#nav li ul li a{
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width:191px; /*----OK*/
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height:28px;/*----OK*/
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color:#FFF; /*()----OK*/
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#nav li ul li a:hover{
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#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .visualClear {display: none;} /*-- hides default wiki settings --*/
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .visualClear {display: none;} /*-- hides default wiki settings --*/
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.annItem li {list-style: none; margin: 5px}
.annItem li {list-style: none; margin: 5px}
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@charset "utf-8";
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body,h1,h2,h3,h4,h5,h6,hr,p,blockquote,dl,dt,dd,ul,ol,li,pre,form,fieldset,legend,button,input,textarea,th,td,figure{
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#apDiv1 { font-family: "微软雅黑";
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html{background-color:#e9ecec}
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.hd-main .navs{padding:0 0 3px 25px;height:45px;float:left}
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.hd-main .navs .app-url .zhaohui .icon{background-position:-250px 0}
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.hd-main .navs .app-url .li .text{height:30px;display:block;font:10px/30px "Microsoft YaHei","Microsoft JhengHei"}
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.hd-main .navs .pulldown-nav em{
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position:absolute;
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left:99px;
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top:18px;
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font-size:0;
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background-position:-89px -45px
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 +
.hd-main .navs .current,.hd-main .navs .current .pulldown-nav{font-weight:bold;color:#FFF;display:inline-block;width:125px;height:49px;background-color:#0f0f0f;text-align:center;line-height:48px}
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.hd-main{padding:0 5px;height:49px;min-width:800px;background:#252525;border-bottom:0}
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.hd-main .info .notice-nu .f-icon{display:inline-block;padding-right:4px;background-position:right -109px;color:#FFF}  /*此处原为#FFF*/
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#apDiv1 {
position:absolute;
position:absolute;
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/*<![CDATA[*/
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</head>
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<!-- ImageReady Slices (middle - 副本.psd) -->
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<body id="" class="">
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<div id="apDiv4">
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<div id="apDiv2">
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   <table width="987" height="216" border="0" align="center" cellpadding="0" cellspacing="0" id="__01">
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  <p><img src="lzuchianmfctt.jpg" width="1366" height="541" /></p>
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  <p>&nbsp;</p>
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  <p>&nbsp;</p>
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  <p>&nbsp;</p>
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  <p>&nbsp;</p>
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  <p>&nbsp;</p>
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</div>
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<body id="" class="">
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<div id="apDiv2b">
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   <p><img src="lzumfctt2.jpg" width="1366" height="542" /></p>
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  <p><img src="bkcolorind.jpg" width="1366" height="482" /></p>
 +
  <p>&nbsp;</p>
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  <p>&nbsp;</p>
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</div>
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<div id="apDiv2">
 +
  <p><img src="lzuchianmfctt.jpg" width="1366" height="541" /></p>
 +
  <p>&nbsp;</p>
 +
  <p>&nbsp;</p>
 +
  <p>&nbsp;</p>
 +
  <p>&nbsp;</p>
 +
  <p>&nbsp;</p>
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</div>
 +
 
 +
 
 +
 
 +
<div id="apDiv3">
 +
  <p>&nbsp;</p>
 +
  <p>&nbsp;</p>
 +
  <div>CONTENTS</div>
 +
  <p>&nbsp;</p>
 +
  <p>&nbsp;</p>
 +
  <p>&nbsp;</p>
 +
  <table width="100%" border="0" align="center">
     <tr>
     <tr>
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       <td colspan="6"><img src="https://static.igem.org/mediawiki/2014/7/77/Newhead_01.jpg" width="987" height="155" alt=""></td>
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       <td width="20%"><p>&nbsp;</p>
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      <p>&nbsp;</p></td>
 +
      <td width="12%"><p><img src="icon_mini/1backgroud.png" width="100" height="100" /></p>
 +
      <p>Background</p></td>
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      <td width="12%"><p><img src="icon_mini/2wetlab.png" width="100" height="100" /></p>
 +
      <p>Wet Lab</p></td>
 +
      <td width="12%"><p><img src="icon_mini/3drylab.png" width="100" height="100" /></p>
 +
      <p>Dry Lab</p></td>
 +
      <td width="12%"><p><img src="icon_mini/4parts.png" width="100" height="100" /></p>
 +
      <p>Parts</p></td>
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      <td width="12%"><p><img src="icon_mini/5humanpractics.png" width="100" height="100" /></p>
 +
      <p>Human Practice</p></td>
 +
      <td width="20%">&nbsp;</td>
     </tr>
     </tr>
     <tr>
     <tr>
-
       <td><img src="https://static.igem.org/mediawiki/2014/e/eb/Newhead_02.jpg" width="402" height="61" alt=""></td>
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       <td width="20%">&nbsp;</td>
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  <p>LanZhou University | 222 TianShui South Road, LanZhou, China, 730000<br>
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      <div> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;ABSTRACT</div>
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          <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" width="57%">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Microbial Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is an ideal approach to solve both pollution problem and energy crisis. However, MFCs still have disadvantages such as hard to determine the contaminants concentrations which is a major drawback for MFCs applications. <br />
 +
            &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;In our study, we found that the electricity produced by MFCs was somewhat related to the substrates concentrations such as P-nitrophenol (PNP) in anode or Chromium (VI) in cathode. Therefore, we hypothesize that a) by using genetic engineered bacteria, the MFC's electricity will be more stable and correlated with substrates concentrations. b) by monitoring MFC's electricity, it will be possible to measure substrates concentrations. <br />
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            &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;In this study, we've designed a novel MFC system. For anode strain, we've cloned a PNP sensor sequence and a riboflavin into Escherichia coli. The recombinants is able to detect PNP and produce riboflavin to boost electrical generation when co-cultured with Shewanella oneidensis. In the cathode, gene codes chromate (VI) reductase Yief was cloned into E.coli. The stability of MFCs has been improved and the electricity generated to correlated with the substrates. Moreover, based on this correlation, we've designed a program which is able to monitor the contaminants concentrations in MFCs. <br /></td>
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Revision as of 23:18, 16 October 2014

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" " http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> LZU-China 2014

 

 

 

 

 

 

 

 

 

 

 

 

 

 

CONTENTS

 

 

 

 

 

Background

Wet Lab

Dry Lab

Parts

Human Practice

  
 

 

 

 

    

 

    
 

Our team

Interlab

Safety

Notebook

Future Work

  

 

 

 

*In order to get better user experience, we recommend the Internet Explorer 10 (or higher version). And the best resolution is 1366 X 768.
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        ABSTRACT
              Microbial Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is an ideal approach to solve both pollution problem and energy crisis. However, MFCs still have disadvantages such as hard to determine the contaminants concentrations which is a major drawback for MFCs applications.
           In our study, we found that the electricity produced by MFCs was somewhat related to the substrates concentrations such as P-nitrophenol (PNP) in anode or Chromium (VI) in cathode. Therefore, we hypothesize that a) by using genetic engineered bacteria, the MFC's electricity will be more stable and correlated with substrates concentrations. b) by monitoring MFC's electricity, it will be possible to measure substrates concentrations.
           In this study, we've designed a novel MFC system. For anode strain, we've cloned a PNP sensor sequence and a riboflavin into Escherichia coli. The recombinants is able to detect PNP and produce riboflavin to boost electrical generation when co-cultured with Shewanella oneidensis. In the cathode, gene codes chromate (VI) reductase Yief was cloned into E.coli. The stability of MFCs has been improved and the electricity generated to correlated with the substrates. Moreover, based on this correlation, we've designed a program which is able to monitor the contaminants concentrations in MFCs.
 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Retrieved from "http://2014.igem.org/Team:LZU-China"