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Team:Cambridge-JIC/Guide/Constructs/Reporters
From 2014.igem.org
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| - | The following fluorescent proteins have been used with consistent results in | + | =Reporters= |
| + | ==Fluorescent proteins== | ||
| + | The following fluorescent proteins have been used with consistent results in Marchantia: | ||
[http://parts.igem.org/Part:BBa_K165005 Venus YFP], MRFP1 and eGFP. | [http://parts.igem.org/Part:BBa_K165005 Venus YFP], MRFP1 and eGFP. | ||
| + | ==Localisation Tags== | ||
A range of localisation tags can be added to enable to distinguish sources of expression and to visualise individual cells and nuclei. These include: | A range of localisation tags can be added to enable to distinguish sources of expression and to visualise individual cells and nuclei. These include: | ||
[http://parts.igem.org/Part:BBa_K1484104 N7] and [http://parts.igem.org/Part:BBa_K1484106 LTI]. | [http://parts.igem.org/Part:BBa_K1484104 N7] and [http://parts.igem.org/Part:BBa_K1484106 LTI]. | ||
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Fluorescent transformants are visible first from 3-5 days after transformation. | Fluorescent transformants are visible first from 3-5 days after transformation. | ||
| + | ==Other reporters== | ||
GUS staining has been successfully used to characterise expression, as seen in in this [http://www.researchgate.net/publication/256611354_Comparison_of_the_MpEF1_and_CaMV35_promoters_for_application_in_Marchantia_polymorpha_overexpression_studies paper] | GUS staining has been successfully used to characterise expression, as seen in in this [http://www.researchgate.net/publication/256611354_Comparison_of_the_MpEF1_and_CaMV35_promoters_for_application_in_Marchantia_polymorpha_overexpression_studies paper] | ||
Revision as of 23:11, 16 October 2014
Contents |
Reporters
Fluorescent proteins
The following fluorescent proteins have been used with consistent results in Marchantia: [http://parts.igem.org/Part:BBa_K165005 Venus YFP], MRFP1 and eGFP.
Localisation Tags
A range of localisation tags can be added to enable to distinguish sources of expression and to visualise individual cells and nuclei. These include: [http://parts.igem.org/Part:BBa_K1484104 N7] and [http://parts.igem.org/Part:BBa_K1484106 LTI].
These can be added instead of the stop codon for the reporters
These are clearly visible under fluorescent microscopes and confocal microscopy. Sample images include:
LTI
Fluorescent transformants are visible first from 3-5 days after transformation.
Other reporters
GUS staining has been successfully used to characterise expression, as seen in in this [http://www.researchgate.net/publication/256611354_Comparison_of_the_MpEF1_and_CaMV35_promoters_for_application_in_Marchantia_polymorpha_overexpression_studies paper]
