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Team:Cambridge-JIC/Guide/Constructs/CodingSequences
From 2014.igem.org
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| - | If possible, codon optimise your sequence for use in plants, or preferably for use with | + | == Coding Sequences == |
| + | If possible, codon optimise your sequence for use in plants, or preferably for use with Marchantia polymorpha. A [[Team:Cambridge-JIC/Marchantia/Codon | codon table]] for Marchantia produced from predicted ORFs is available on this wiki. | ||
There is evidence of a considerable number splice sites in the MP genome, so there is no guarantee that a coding sequence that works in other chassis will be correctly translated in Marchantia. | There is evidence of a considerable number splice sites in the MP genome, so there is no guarantee that a coding sequence that works in other chassis will be correctly translated in Marchantia. | ||
Best to try! | Best to try! | ||
Revision as of 22:56, 16 October 2014
Coding Sequences
If possible, codon optimise your sequence for use in plants, or preferably for use with Marchantia polymorpha. A codon table for Marchantia produced from predicted ORFs is available on this wiki.
There is evidence of a considerable number splice sites in the MP genome, so there is no guarantee that a coding sequence that works in other chassis will be correctly translated in Marchantia.
Best to try!
