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Team:Cambridge-JIC/Guide/Constructs/CodingSequences
From 2014.igem.org
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(Created page with "If possible, codon optimise your sequence for use in plants, or preferably for use with MP. A [codon table] for MP produced from predicted ORFs is available on this wiki. There ...") |
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| - | If possible, codon optimise your sequence for use in plants, or preferably for use with MP. A [codon table] for | + | If possible, codon optimise your sequence for use in plants, or preferably for use with MP. A [[Team:Cambridge-JIC/Marchantia/Codon | codon table}] for Marchantia produced from predicted ORFs is available on this wiki. |
| - | There is evidence of considerable | + | There is evidence of a considerable number splice sites in the MP genome, so there is no guarantee that a coding sequence that works in other chassis will be correctly translated in Marchantia. |
| + | |||
| + | Best to try! | ||
Revision as of 22:56, 16 October 2014
If possible, codon optimise your sequence for use in plants, or preferably for use with MP. A [[Team:Cambridge-JIC/Marchantia/Codon | codon table}] for Marchantia produced from predicted ORFs is available on this wiki.
There is evidence of a considerable number splice sites in the MP genome, so there is no guarantee that a coding sequence that works in other chassis will be correctly translated in Marchantia.
Best to try!
