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Team:Cambridge-JIC/Guide/Constructs/Promoters
From 2014.igem.org
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| - | The two most reliable constitutive promoters in MP are the [http://parts.igem.org/Part:BBa_K509000 | + | The two most reliable constitutive promoters in MP are the [http://parts.igem.org/Part:BBa_K509000 Cauliflower Mosaic Virus 35S] and [http://parts.igem.org/Part:BBa_K1484214 MpEF1a]. They have different expression patterns on the thallus, which are summarised in this [http://www.researchgate.net/publication/256611354_Comparison_of_the_MpEF1_and_CaMV35_promoters_for_application_in_Marchantia_polymorpha_overexpression_studies paper]. |
The parts above in the registry include the 5'UTRs. If using one of the above, simply have your ATG adjacent to the promoter. | The parts above in the registry include the 5'UTRs. If using one of the above, simply have your ATG adjacent to the promoter. | ||
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When trying to express any protein new to Marchantia, it is recommended that you use one of the above in at least one of your constructs. Expression seems to be different in Marchantia from other model plants, so it is important to have a reliable promoter. | When trying to express any protein new to Marchantia, it is recommended that you use one of the above in at least one of your constructs. Expression seems to be different in Marchantia from other model plants, so it is important to have a reliable promoter. | ||
| - | Another option is the [http://parts.igem.org/Part:BBa_K1484314 | + | Another option is the [http://parts.igem.org/Part:BBa_K1484314 Gal4 Amplification cassette], which can be used to amplify your signal, when used in conjunction with a promoter. |
The annotation of the Marchantia genome is underway, so there are other possibilities for promoters being tested currently. | The annotation of the Marchantia genome is underway, so there are other possibilities for promoters being tested currently. | ||
Revision as of 22:54, 16 October 2014
The two most reliable constitutive promoters in MP are the [http://parts.igem.org/Part:BBa_K509000 Cauliflower Mosaic Virus 35S] and [http://parts.igem.org/Part:BBa_K1484214 MpEF1a]. They have different expression patterns on the thallus, which are summarised in this [http://www.researchgate.net/publication/256611354_Comparison_of_the_MpEF1_and_CaMV35_promoters_for_application_in_Marchantia_polymorpha_overexpression_studies paper].
The parts above in the registry include the 5'UTRs. If using one of the above, simply have your ATG adjacent to the promoter.
Other plant promoters may or may not produce results in Marchantia.
When trying to express any protein new to Marchantia, it is recommended that you use one of the above in at least one of your constructs. Expression seems to be different in Marchantia from other model plants, so it is important to have a reliable promoter.
Another option is the [http://parts.igem.org/Part:BBa_K1484314 Gal4 Amplification cassette], which can be used to amplify your signal, when used in conjunction with a promoter.
The annotation of the Marchantia genome is underway, so there are other possibilities for promoters being tested currently.
