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Team:Evry/Interlab Study/09-14-2014
From 2014.igem.org
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pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation 16h at 37°C. | pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation 16h at 37°C. | ||
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| - | <center>GEL 1: 1% agarose gel. PCR product of | + | <center>GEL 1: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23114 construction. </center> |
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Revision as of 22:51, 16 October 2014
Construction n°1: PSB1C3 with I20260
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
Other constructions of the Anderson library of constitutive promoters
PSB1C3+E0240+J23101:
Measure of the cells' growth and fluorecence on TECAN infiniteM200.
TECAN FAILED
PSB1C3+E0240+J23106:
pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation 16h at 37°C.
PSB1C3+E0240+J23114, PSB1C3+E0240+J23115 and PSB1C3+E0240+J23116:
- PCR colony of 5 colonies for each construction following protocol table 1 and 2.
GEL 1: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23114 construction.
GEL 2: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23116 and PSB1C3+E0240+J23115 constructions.
