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Team:Evry/Interlab Study/09-15-2014
From 2014.igem.org
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| - | + | <i>PSB1C3+E0240+J23117</i>, <i>PSB1C3+E0240+J23119</i>: <br> | |
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| - | <li> PCR colony of | + | <li> PCR colony of 6 colonies for each construction following protocol table 1 and 2. |
Revision as of 22:50, 16 October 2014
Construction n°1: PSB1C3 with I20260
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
Pre-culture from glycerol stock in LB+Cam (1:1000). Incubation 16h-18h at 37°C.
Other constructions of the Anderson library of constitutive promoters
PSB1C3+E0240+J23100:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23106:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23117, PSB1C3+E0240+J23119:
- PCR colony of 6 colonies for each construction following protocol table 1 and 2.
GEL 1: 1% agarose gel. PCR product of 6 colonies of the PSB1C3+E0240+J23117 and PSB1C3+E0240+J23119 constructions.
