Team:UFAM Brazil/8-8-2014

From 2014.igem.org

(Difference between revisions)
 
Line 12: Line 12:
<p>
<p>
-
Heeeyy !! Today remade the plasmid extraction with kit JM110, and DH5α with Biobrick for bioremediation and essential Biobrick.
+
Heeeyy!! Today we made plasmid extraction with kit JM110, and DH5α with Biobrick for bioremediation and essential Biobrick.
</p>
</p>
Line 21: Line 21:
<p><b>Eletrophoretical profile of samples</b></p>
<p><b>Eletrophoretical profile of samples</b></p>
-
</td></tr>
+
</td></tr>scho
<tr><td colspan="3" align="center">
<tr><td colspan="3" align="center">
Line 29: Line 29:
<tr><td colspan="3"  align="justify">
<tr><td colspan="3"  align="justify">
-
<p style="margin-left:300px">1. Biobrick essencial extraído de DH5α (1)</p>
+
<p style="margin-left:300px">1. Biobrick Essential extracted from DH5α (1)</p>
-
<p style="margin-left:300px">2. Biobrick essencial extraído de DH5α (2)</p>
+
<p style="margin-left:300px">2. Biobrick Essential extracted from (2)</p>
-
<p style="margin-left:300px">3. Biobrick para biorremediação extraído de JM110 (1)</p>
+
<p style="margin-left:300px">3. Biobrick for bioremediation exctracted from JM110 (1)</p>
-
<p style="margin-left:300px">4. Biobrick para biorremediação extraído de JM110 (2)</p>
+
<p style="margin-left:300px">4. Biobrick for bioremediation exctracted from JM110 (2)</p>
<p>
<p>
-
  Após a extração, digerimos as amostras com EcoRI e PstI afim de analisar o tamanho do fragmento do Biobrick essencial e Biobrick para biorremediação. Utilizamos o seguinte sistema para digestão enzimática:  
+
  After extraction, the samples were digested with EcoRI and PstI to check fragment's size of Essential Biobrick and Biorremediation Biobrick. For this we used the following system for enzymatic digestion:  
</p>
</p>
</td></tr>
</td></tr>
Line 47: Line 47:
<p>
<p>
-
  The digestion lasted 2 hours at 37°C. We applied the gel with 0.8% of agarose  the total volume of each sample.
+
  The digestion took 2 hours at 37°C. We applied the total volume of each sample in agarose gel 0.8%.
</p>
</p>
Line 68: Line 68:
<p>
<p>
-
Hurray!!!  The digestion has released fragments that proved the viability our beloved biobricks. Bioremediation Biobrick has  1.823  base  pairs and Essential Biobrickhas 1.234 base pairs,that’s why BB_Biorremediation sits above BB_Essential in the gel. =)
+
Hurray!!!  The digestion released fragments. It proves the viability of our beloved biobricks! Bioremediation Biobrick has  1.823  base  pairs and Essential Biobrick has 1.234 base pairs,that’s why BB_Biorremediation sits above BB_Essential in the gel. =)
</p>
</p>
-
<p> We have also analyzed samples 5-6-7-8 that didn’t show digestion or it showed partial digestion (Either no presented digestion or not presented partial digestion). </p>
+
<p> We have also analyzed samples 5-6-7-8 that wasn't digested or showed partial digestion (Either didn't present digestion or partial digestion). </p>
</td></tr>
</td></tr>

Latest revision as of 22:10, 16 October 2014

scho

08/08/2014

Heeeyy!! Today we made plasmid extraction with kit JM110, and DH5α with Biobrick for bioremediation and essential Biobrick.

Eletrophoretical profile of samples

1. Biobrick Essential extracted from DH5α (1)

2. Biobrick Essential extracted from (2)

3. Biobrick for bioremediation exctracted from JM110 (1)

4. Biobrick for bioremediation exctracted from JM110 (2)

After extraction, the samples were digested with EcoRI and PstI to check fragment's size of Essential Biobrick and Biorremediation Biobrick. For this we used the following system for enzymatic digestion:

The digestion took 2 hours at 37°C. We applied the total volume of each sample in agarose gel 0.8%.

Eletrophoretical profile of the digested samples:

1. Bioremediation Biobrick not digested.

2. Bioremediation Biobrick digested with EcoRI and PstI

3. Essential Biobrick not digested.

4. Essential Biobrick digested with EcoRI and PstI.

5-6-7-8. Plasmids did not show a good extraction

Hurray!!! The digestion released fragments. It proves the viability of our beloved biobricks! Bioremediation Biobrick has 1.823 base pairs and Essential Biobrick has 1.234 base pairs,that’s why BB_Biorremediation sits above BB_Essential in the gel. =)

We have also analyzed samples 5-6-7-8 that wasn't digested or showed partial digestion (Either didn't present digestion or partial digestion).

Back Next

Retrieved from "http://2014.igem.org/Team:UFAM_Brazil/8-8-2014"