Team:INSA-Lyon/Results

From 2014.igem.org

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<p>What about chelation ? <br/>
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<div align="justify">What about chelation ? <br/>
Nickel(II) chelated for each of the constructions (WT, HIS1,  HIS2) is evaluated by using dimethylglyoxime (DMG) as the precipitating reagent. This is achieved by using absorbing properties of DMG-Ni(II) pink-colored complex (peak absorption at 554nm). <br/>
Nickel(II) chelated for each of the constructions (WT, HIS1,  HIS2) is evaluated by using dimethylglyoxime (DMG) as the precipitating reagent. This is achieved by using absorbing properties of DMG-Ni(II) pink-colored complex (peak absorption at 554nm). <br/>
Firstly, a calibration set of Nickel and DMG was done. <br/>
Firstly, a calibration set of Nickel and DMG was done. <br/>
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Taken together, these results show that the constructed Strain CsgA- with part BBa_K1404008 chelates twice more than strain CsgA- with part BBa_K1404007. That means that two Histags on C-term are twice more effective than one. Moreover, one histag allows a better chelation than none, but not a really significative one.  Significant differences are indicated using lowercase letters, and different letters indicate significant differences (Tukey’s test, p < 0.05). Error bars represent standard deviations.  
Taken together, these results show that the constructed Strain CsgA- with part BBa_K1404008 chelates twice more than strain CsgA- with part BBa_K1404007. That means that two Histags on C-term are twice more effective than one. Moreover, one histag allows a better chelation than none, but not a really significative one.  Significant differences are indicated using lowercase letters, and different letters indicate significant differences (Tukey’s test, p < 0.05). Error bars represent standard deviations.  
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Revision as of 21:52, 16 October 2014

Curly'on - IGEM 2014 INSA-LYON

  • Biofilm production


  • Nickel chelation


  • Survival after UV and high temperature exposure


  • Promoter optimization and characterization