Team:Groningen/Template/MODULE/projects/parts/detection

From 2014.igem.org

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Detection system for <i> Pseudonomas aeruginosa</i> 
 
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<a href="http://parts.igem.org/Part:BBa_K1365997">BBa_K1365997</a> , <a href="http://parts.igem.org/Part:BBa_K1365998">BBa_K1365998</a> &
 
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<a href="http://parts.igem.org/Part:BBa_K1365999">BBa_K1365999</a>
 
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To give our LactoAid the ability to detect<i> Pseudonomas aeruginosa</i> we assembled a new biobrick. For the detection we adopted a widely used principle for detection, namely detection of quorum molecules. <i> P. aeruginosa produces</i> AHLs for communication [Source] and we build an <a href="http://parts.igem.org/Part:BBa_K1365997"> AHL sensor (BBa_K1365997)</a> designed for<i> L. lactis</i>.
 
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To test the how well our LactoAid detects <i>Pseudonomas aeruginosa</i>we build a construct which has the <a href="http://parts.igem.org/Part:BBa_K1365998">AHL senor ability coupled with an super fold GFP (BBa_K1365998)</a> .
 
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The third part made for the detection is an <a href="http://parts.igem.org/Part:BBa_K1365999">AHL generator (BBa_K1365999)</a>. Many iGEM teams are working with AHL sensing, either to detected <i> Pseudonomas aeruginosa</i>or for an regulatory mechanism. Our AHL generator produces AHLs which enables other teams to produce AHLs and test their sensors without the hassle of working with the pathogenic bacterium itself. 
 
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Detection system for <i> Staphylococcus aureus</i> 
 
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The third part made for the detection is an <a href="http://parts.igem.org/Part:BBa_K1365999">AHL generator (BBa_K1365999)</a>. Many iGEM teams are working with AHL sensing, either to detected <i> Pseudonomas aeruginosa</i>or for an regulatory mechanism. Our AHL generator produces AHLs which enables other teams to produce AHLs and test their sensors without the hassle of working with the pathogenic bacterium itself. 
 
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Revision as of 21:32, 16 October 2014