Team:Cambridge-JIC/Guide/Materials/Checklist

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Revision as of 21:21, 16 October 2014

      • Checklist

This checklist contains everything you will need for setting up transformation

        • DNA

- A helper plasmid for agrobacterium mediated transformation - Your T-DNA plasmid for agrobacterium mediated transformation [see the MoClo plate].

- Competent E-Coli (chemically/electro) - Electrocompetent agrobacteria

        • Marchantia

- Marchantia sporangia OR - Male and female Marchantia polymorpha [from the garden]

        • Misc

- Gamborg's B5 salt mix - LB/SOC for bacterial transformation and growth - 100mM Acetosyringone

        • Equipment

- Ventilated cupboard/room/box under full illumination [larger space = more transformations]

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