3/13/14
- Digested pBR322 with HindIII.
- Double digested pBR322 with HindIII and EcoRI.
3/27/14
- Ran digests from 3/13/14 on gel.
- Excised gel fragments with plasmid in them.
- Purified gel fragments.
4/10/14
Phosphatase treated the digested and double digested pBR322 from 3/13/14.
4/17/14
- Digested UV, HSP, and GFP g-blocks with HindIII and EcoRI.
- Performed five ligations:
- pBR322 with UV
- pBR322 with HSP
- pBR322 with GFP
- UV with GFP
- HSP with GFP
4/24/14
- Purified UV+GFP and HSP+GFP ligations.
- Digested the two ligations with HindIII.
- Ligated each one into pBR322.
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