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Team:Groningen/Template/MODULE/Notebook/toolbox/week11
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Revision as of 20:38, 16 October 2014
22 - 28 September
SEQUENCING RESULTS
A PCR was done to generate fragments for assembling genes that had
illegal restriction sites. Every fragment was succesfully amplified.
The fragments were purified using the GeneJET PCR clean up kit.
were not fully clean, so only the fragments for
the combined BioBrick of NisF, NisE and NisG was made using Gibson
assembly. The transformants that contained pSB1C3 with NisFEG were
tested using colony PCR. No positive transformants were found.
