Team:Austin Texas/kit

From 2014.igem.org

(Difference between revisions)
(Experimental Design and Method)
(Experimental Design and Method)
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*'''pFRYC''' is the control plasmid and contains an IPTG-induced reporter system and a kanamycin resistance gene.  The reporter system is composed of RFP and sfGFP fused via a linker sequence between the two.
*'''pFRYC''' is the control plasmid and contains an IPTG-induced reporter system and a kanamycin resistance gene.  The reporter system is composed of RFP and sfGFP fused via a linker sequence between the two.
*'''pFRY''' is the experimental reporter plasmid and is nearly identical to pFRYC with the exception that its linker sequence contains an amber codon (star in figure 1) in the middle of the linker, whereas pFRYC contains a tyrosine codon in the same location.
*'''pFRY''' is the experimental reporter plasmid and is nearly identical to pFRYC with the exception that its linker sequence contains an amber codon (star in figure 1) in the middle of the linker, whereas pFRYC contains a tyrosine codon in the same location.
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Revision as of 20:03, 16 October 2014