Team:Freiburg/Content/Results/BioBrick
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<section> | <section> | ||
<h3>Constitutive Expression</h3> | <h3>Constitutive Expression</h3> | ||
- | <h4>p14rz_003</h4> | + | <h4>p14rz_003: pcDNA_SLC7A1_GFP</h4> |
<div class="row category-row"> | <div class="row category-row"> | ||
<div class="col-sm-6"> | <div class="col-sm-6"> | ||
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<img src="https://static.igem.org/mediawiki/2014/7/7b/Freiburg2014_p14rz_003_pcDNA_SLC7A1_GFP.png"> <!-- Thumbnail --> | <img src="https://static.igem.org/mediawiki/2014/7/7b/Freiburg2014_p14rz_003_pcDNA_SLC7A1_GFP.png"> <!-- Thumbnail --> | ||
</a> | </a> | ||
- | |||
- | |||
- | |||
</figure> | </figure> | ||
</div> | </div> | ||
</div> | </div> | ||
- | <h4>p14rz_004</h4> | + | <h4>p14rz_004: pcDNA_SLC7A1_HA</h4> |
<div class="row category-row"> | <div class="row category-row"> | ||
<div class="col-sm-6"> | <div class="col-sm-6"> | ||
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<img src="https://static.igem.org/mediawiki/2014/0/01/Freiburg2014_p14rz_004_pcDNA_SLC7A1_HA.png"> <!-- Thumbnail --> | <img src="https://static.igem.org/mediawiki/2014/0/01/Freiburg2014_p14rz_004_pcDNA_SLC7A1_HA.png"> <!-- Thumbnail --> | ||
</a> | </a> | ||
- | |||
- | |||
- | |||
</figure> | </figure> | ||
</div> | </div> | ||
</div> | </div> | ||
- | <h4>p14rz_005</h4> | + | <h4>p14rz_005: pcDNA_SLC7A1_mCherry</h4> |
<div class="row category-row"> | <div class="row category-row"> | ||
<div class="col-sm-6"> | <div class="col-sm-6"> | ||
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<img src="https://static.igem.org/mediawiki/2014/2/21/Freiburg2014_p14rz_005_pcDNA_SLC7A1_mCherry.png"> <!-- Thumbnail --> | <img src="https://static.igem.org/mediawiki/2014/2/21/Freiburg2014_p14rz_005_pcDNA_SLC7A1_mCherry.png"> <!-- Thumbnail --> | ||
</a> | </a> | ||
- | |||
- | |||
- | |||
</figure> | </figure> | ||
</div> | </div> | ||
</div> | </div> | ||
- | <h4>p14rz_006</h4> | + | <h4>p14rz_006: pcDNA_SLC7A1_HA_p2a_mCherry</h4> |
<div class="row category-row"> | <div class="row category-row"> | ||
<div class="col-sm-6"> | <div class="col-sm-6"> | ||
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<img src="https://static.igem.org/mediawiki/2014/d/d4/Freiburg2014_p14rz_006_pcDNA_SLC7A1_HA_p2a_mCherry.png"> <!-- Thumbnail --> | <img src="https://static.igem.org/mediawiki/2014/d/d4/Freiburg2014_p14rz_006_pcDNA_SLC7A1_HA_p2a_mCherry.png"> <!-- Thumbnail --> | ||
</a> | </a> | ||
- | |||
- | |||
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</figure> | </figure> | ||
</div> | </div> | ||
</div> | </div> | ||
- | <h4>p14rz_007</h4> | + | <h4>p14rz_007: pcDNA_SLC7A1_HA_mCherry</h4> |
<div class="row category-row"> | <div class="row category-row"> | ||
<div class="col-sm-6"> | <div class="col-sm-6"> | ||
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<img src="https://static.igem.org/mediawiki/2014/a/a2/Freiburg2014_p14rz_007_pcDNA_SLC7A1_HA_mCherry.png"> <!-- Thumbnail --> | <img src="https://static.igem.org/mediawiki/2014/a/a2/Freiburg2014_p14rz_007_pcDNA_SLC7A1_HA_mCherry.png"> <!-- Thumbnail --> | ||
</a> | </a> | ||
- | |||
- | |||
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</figure> | </figure> | ||
</div> | </div> | ||
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<section> | <section> | ||
<h3>Red Light induced</h3> | <h3>Red Light induced</h3> | ||
- | <h4>p14rz_010</h4> | + | <h4>p14rz_010: TetO13_CMVmin_SLC7A1_HA_p2a_mCherry</h4> |
- | <figure> | + | <div class="row category-row"> |
+ | <div class="col-sm-6"> | ||
+ | <p>- bla</p> | ||
+ | <p>- blaII</p> | ||
+ | </div> | ||
+ | <div class="col-sm-6"> | ||
+ | <figure> | ||
<a href="https://static.igem.org/mediawiki/2014/6/6b/Freiburg2014_p14rz_010_TetO13_CMVmin_SLC7A1_HA_p2a_mCherry.png"> <!-- ORGINAL --> | <a href="https://static.igem.org/mediawiki/2014/6/6b/Freiburg2014_p14rz_010_TetO13_CMVmin_SLC7A1_HA_p2a_mCherry.png"> <!-- ORGINAL --> | ||
<img src="https://static.igem.org/mediawiki/2014/6/6b/Freiburg2014_p14rz_010_TetO13_CMVmin_SLC7A1_HA_p2a_mCherry.png"> <!-- Thumbnail --> | <img src="https://static.igem.org/mediawiki/2014/6/6b/Freiburg2014_p14rz_010_TetO13_CMVmin_SLC7A1_HA_p2a_mCherry.png"> <!-- Thumbnail --> | ||
</a> | </a> | ||
- | < | + | </figure> |
- | + | </div> | |
- | + | </div> | |
- | + | <h4>p14rz_011: TetO13_CMVmin_SLC7A1_HA_mCherry</h4> | |
- | < | + | <div class="row category-row"> |
- | + | <div class="col-sm-6"> | |
- | + | <p>- bla</p> | |
+ | <p>- blaII</p> | ||
+ | </div> | ||
+ | <div class="col-sm-6"> | ||
+ | <figure> | ||
+ | <a href="https://static.igem.org/mediawiki/2014/f/f6/Freiburg2014_p14rz_011_TetO13_CMVmin_SLC7A1_HA_mCherry.png"> <!-- ORGINAL --> | ||
<img src="https://static.igem.org/mediawiki/2014/f/f6/Freiburg2014_p14rz_011_TetO13_CMVmin_SLC7A1_HA_mCherry.png"> <!-- Thumbnail --> | <img src="https://static.igem.org/mediawiki/2014/f/f6/Freiburg2014_p14rz_011_TetO13_CMVmin_SLC7A1_HA_mCherry.png"> <!-- Thumbnail --> | ||
- | + | </a> | |
- | + | </figure> | |
- | + | </div> | |
- | </ | + | </div> |
- | </ | + | |
</section> | </section> | ||
<section> | <section> | ||
<h3>Blue Light induced</h3> | <h3>Blue Light induced</h3> | ||
- | <h4>p14rz_008</h4> | + | <h4>p14rz_008: Gal4_SLC7A1</h4> |
- | < | + | <div class="row category-row"> |
- | <a href="https://static.igem.org/mediawiki/2014/5/50/Freiburg2014_p14rz_008_Gal4_SLC7A1.png"> <!-- ORGINAL --> | + | <div class="col-sm-6"> |
+ | <p>- bla</p> | ||
+ | <p>- blaII</p> | ||
+ | </div> | ||
+ | <div class="col-sm-6"> | ||
+ | <figure> | ||
+ | <a href="https://static.igem.org/mediawiki/2014/5/50/Freiburg2014_p14rz_008_Gal4_SLC7A1.png"> <!-- ORGINAL --> | ||
<img src="https://static.igem.org/mediawiki/2014/5/50/Freiburg2014_p14rz_008_Gal4_SLC7A1.png"> <!-- Thumbnail --> | <img src="https://static.igem.org/mediawiki/2014/5/50/Freiburg2014_p14rz_008_Gal4_SLC7A1.png"> <!-- Thumbnail --> | ||
- | + | </a> | |
- | < | + | </figure> |
- | + | </div> | |
- | + | </div> | |
- | + | <h4>p14rz_009: Gal4_SLC7A1_HA</h4> | |
- | < | + | <div class="row category-row"> |
- | + | <div class="col-sm-6"> | |
- | + | <p>- bla</p> | |
+ | <p>- blaII</p> | ||
+ | </div> | ||
+ | <div class="col-sm-6"> | ||
+ | <figure> | ||
+ | <a href="https://static.igem.org/mediawiki/2014/f/f5/Freiburg2014_p14rz_009_Gal4_SLC7A1_HA.png"> <!-- ORGINAL --> | ||
<img src="https://static.igem.org/mediawiki/2014/f/f5/Freiburg2014_p14rz_009_Gal4_SLC7A1_HA.png"> <!-- Thumbnail --> | <img src="https://static.igem.org/mediawiki/2014/f/f5/Freiburg2014_p14rz_009_Gal4_SLC7A1_HA.png"> <!-- Thumbnail --> | ||
- | + | </a> | |
- | + | </figure> | |
- | + | </div> | |
- | </ | + | </div> |
- | </ | + | |
</section> | </section> | ||
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<h2 id="Plasmid-Light-Systems">Light System</h2> | <h2 id="Plasmid-Light-Systems">Light System</h2> | ||
<h3>Red Light</h3> | <h3>Red Light</h3> | ||
+ | <figure> | ||
+ | <a href="https://static.igem.org/mediawiki/2014/e/e7/2014Freiburg_PKM292.jpg"> <!-- ORGINAL --> | ||
+ | <img src="https://static.igem.org/mediawiki/2014/e/e7/2014Freiburg_PKM292.jpg"> <!-- Thumbnail --> | ||
+ | </a> | ||
+ | </figure> | ||
<h3>Blue Light</h3> | <h3>Blue Light</h3> | ||
<h3>UV Light</h3> | <h3>UV Light</h3> | ||
- | <h4>p14ls_001</h4> | + | <h4>p14ls_001: COP1-VP16_IRES_TetR-UVR8</h4> |
- | < | + | <div class="row category-row"> |
- | <a href="https://static.igem.org/mediawiki/2014/f/f5/Freiburg2014_p14ls_001_COP1-VP16_IRES_TetR-UVR8.png"> <!-- ORGINAL --> | + | <div class="col-sm-6"> |
+ | <p>- bla</p> | ||
+ | <p>- blaII</p> | ||
+ | </div> | ||
+ | <div class="col-sm-6"> | ||
+ | <figure> | ||
+ | <a href="https://static.igem.org/mediawiki/2014/f/f5/Freiburg2014_p14ls_001_COP1-VP16_IRES_TetR-UVR8.png"> <!-- ORGINAL --> | ||
<img src="https://static.igem.org/mediawiki/2014/f/f5/Freiburg2014_p14ls_001_COP1-VP16_IRES_TetR-UVR8.png"> <!-- Thumbnail --> | <img src="https://static.igem.org/mediawiki/2014/f/f5/Freiburg2014_p14ls_001_COP1-VP16_IRES_TetR-UVR8.png"> <!-- Thumbnail --> | ||
- | + | </a> | |
- | < | + | </figure> |
- | + | </div> | |
- | + | </div> | |
- | + | <h4>p14ls_002: Gal4_mCherry</h4> | |
- | < | + | <div class="row category-row"> |
- | + | <div class="col-sm-6"> | |
- | + | <p>- bla</p> | |
+ | <p>- blaII</p> | ||
+ | </div> | ||
+ | <div class="col-sm-6"> | ||
+ | <figure> | ||
+ | <a href="https://static.igem.org/mediawiki/2014/9/9f/Freiburg2014_p14ls_002_Gal4_mCherry.png"> <!-- ORGINAL --> | ||
<img src="https://static.igem.org/mediawiki/2014/9/9f/Freiburg2014_p14ls_002_Gal4_mCherry.png"> <!-- Thumbnail --> | <img src="https://static.igem.org/mediawiki/2014/9/9f/Freiburg2014_p14ls_002_Gal4_mCherry.png"> <!-- Thumbnail --> | ||
- | + | </a> | |
- | + | </figure> | |
- | + | </div> | |
- | </ | + | </div> |
- | </ | + | |
</section> | </section> | ||
Revision as of 19:29, 16 October 2014
Bio Bricks
BBa_K1470000: mCat-1
mCat-1 is the receptor we introduced to human cells to be able to infect them using the MuLV
BBa_K1470001: Puromycin N-acetyl-transferase
Puromycin N-acetyl-transferase is an enzyme which we used for selecting stable eucaryotic cell lines. Because of the blocked protein biosynthesis more than 99% of uninfected cells will be dead within two days.
BBa_K1470002: Galactose-induced gene 4 (Gal4BD)
Galactose-induced gene 4 (Gal4BD) is used to investigate gene expression levels.
BBa_K1470003: Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE)
Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE) is a regulatory RNA sequence, which promotes viral gene expression.
BBa_K1470004: Secreted Alkaline Phosphatase (SEAP)
Secreted Alkaline Phosphatase (SEAP) is used in cell culture assays to quantify gene expression.
BBa_K1470005: engineered PDZ domain (ePDZ)
engineered PDZ domain (ePDZ) is an important part of the blue light system.
BBa_K1470006: The Viral Vector (pMIG)
The Viral Vector (pMIG) is the plasmid which is used to tranfect Phoenix cells with the gene to be packed into viral particles. It contains two LTRs and a multiple cloning site.
BBa_K1470007: Cas9-Nickase
Cas9-Nickaseis a Cas9 without iGEM restrction-sites but full catalytic activity.
Plasmids
mCat-1 Plasmids
Constitutive Expression
p14rz_003: pcDNA_SLC7A1_GFP
- bla
- blaII
p14rz_004: pcDNA_SLC7A1_HA
- bla
- blaII
p14rz_005: pcDNA_SLC7A1_mCherry
- bla
- blaII
p14rz_006: pcDNA_SLC7A1_HA_p2a_mCherry
- bla
- blaII
p14rz_007: pcDNA_SLC7A1_HA_mCherry
- bla
- blaII
Red Light induced
p14rz_010: TetO13_CMVmin_SLC7A1_HA_p2a_mCherry
- bla
- blaII
p14rz_011: TetO13_CMVmin_SLC7A1_HA_mCherry
- bla
- blaII
Blue Light induced
p14rz_008: Gal4_SLC7A1
- bla
- blaII
p14rz_009: Gal4_SLC7A1_HA
- bla
- blaII
Light System
Red Light
Blue Light
UV Light
p14ls_001: COP1-VP16_IRES_TetR-UVR8
- bla
- blaII
p14ls_002: Gal4_mCherry
- bla
- blaII