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Team:Freiburg/Content/Results/BioBrick
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<section> | <section> | ||
<h4>BBa_K1470000: mCat-1</h4> | <h4>BBa_K1470000: mCat-1</h4> | ||
| - | <p><a href="http://parts.igem.org/Part:BBa_K1470000">mCat-1</a> is the | + | <p><a href="http://parts.igem.org/Part:BBa_K1470000">mCat-1</a> is the receptor we introduced to human cells to be able to infect them using the MuLV </p> |
</section> | </section> | ||
<section> | <section> | ||
<h4>BBa_K1470001: Puromycin N-acetyl-transferase</h4> | <h4>BBa_K1470001: Puromycin N-acetyl-transferase</h4> | ||
| - | <p><a href="http://parts.igem.org/Part:BBa_K1470001">Puromycin N-acetyl-transferase</a></p> | + | <p><a href="http://parts.igem.org/Part:BBa_K1470001">Puromycin N-acetyl-transferase</a> is an enzyme which we used for selecting stable eucaryotic cell lines. Because of the blocked protein biosynthesis more than 99% of uninfected cells will be dead within two days. </p> |
</section> | </section> | ||
<section> | <section> | ||
<h4>BBa_K1470002: Galactose-induced gene 4 (Gal4BD)</h4> | <h4>BBa_K1470002: Galactose-induced gene 4 (Gal4BD)</h4> | ||
| - | <p><a href="http://parts.igem.org/Part:BBa_K1470002">Galactose-induced gene 4 (Gal4BD)</a></p> | + | <p><a href="http://parts.igem.org/Part:BBa_K1470002">Galactose-induced gene 4 (Gal4BD)</a> is used to investigate gene expression levels.</p> |
</section> | </section> | ||
<section> | <section> | ||
| - | <h4>Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE)</h4> | + | <h4>BBa_K1470003: Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE)</h4> |
| - | <p><a href="http://parts.igem.org/Part:BBa_K1470003">Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE)</a></p> | + | <p><a href="http://parts.igem.org/Part:BBa_K1470003">Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE)</a> is a regulatory RNA sequence, which promotes viral gene expression.</p> |
</section> | </section> | ||
<section> | <section> | ||
Revision as of 19:12, 16 October 2014
Bio Bricks
BBa_K1470000: mCat-1
mCat-1 is the receptor we introduced to human cells to be able to infect them using the MuLV
BBa_K1470001: Puromycin N-acetyl-transferase
Puromycin N-acetyl-transferase is an enzyme which we used for selecting stable eucaryotic cell lines. Because of the blocked protein biosynthesis more than 99% of uninfected cells will be dead within two days.
BBa_K1470002: Galactose-induced gene 4 (Gal4BD)
Galactose-induced gene 4 (Gal4BD) is used to investigate gene expression levels.
BBa_K1470003: Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE)
Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE) is a regulatory RNA sequence, which promotes viral gene expression.
BBa_K1470004: Secreted Alkaline Phosphatase (SEAP)
BBa_K1470005: engineered PDZ domain (ePDZ)
BBa_K1470006: The Viral Vector (pMIG)
BBa_K1470007: Cas9-Nickase
Plasmids
mCat-1 Plasmids
Constitutive Expression
p14rz_003
- bla
- blaII
p14rz_003: pcDNA_SLC7A1_GFP
p14rz_004
- bla
- blaII
Freiburg2014_p14rz_004_pcDNA_SLC7A1_HA.png
p14rz_005
- bla
- blaII
Freiburg2014_p14rz_005_pcDNA_SLC7A1_mCherry.png
p14rz_006
- bla
- blaII
Freiburg2014_p14rz_006_pcDNA_SLC7A1_HA_p2a_mCherry.png
p14rz_007
- bla
- blaII
Freiburg2014_p14rz_007_pcDNA_SLC7A1_HA_mCherry.png
Red Light induced
p14rz_010
Freiburg2014_p14rz_010_TetO13_CMVmin_SLC7A1_HA_p2a_mCherry.png
p14rz_011
Freiburg2014_p14rz_011_TetO13_CMVmin_SLC7A1_HA_mCherry.png
Blue Light induced
p14rz_008
Freiburg2014_p14rz_008_Gal4_SLC7A1.png
p14rz_009
Freiburg2014_p14rz_009_Gal4_SLC7A1_HA.png
Light System
Red Light
Blue Light
UV Light
p14ls_001
p14ls_001_COP1-VP16_IRES_TetR-UVR8
p14ls_002
Freiburg2014_p14ls_002_Gal4_mCherry
