From 2014.igem.org
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Revision as of 18:01, 16 October 2014
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Attributions |
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We would like to thank all the people who came to our progress presentations and gave useful feedback. |
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Instructors support |
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Experiment |
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Bobo, Regina, Denise, Eating
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Contributions |
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Transformation ( Cas9 in pSB1C3 into DH5α)
Extract plasmid with pSB1C3 from DH5α.
Extract plasmid pSB1C3 with BBa_I13521、BBa_ K914003、BBa_ K1218011.
Use Enzyme digestion (PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.
Purification of RFP sequence from pSB1C3 Prepare Cm. plate、LB. plate.
Ligation of RFP and pBluescript SK(-).
Transformation clone RFP/pBluescript SK(-) to DH5α.
Extract plasmid clone RFP/pBluescript SK(-). |
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Modeling |
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House, Bobo, Chao-Di Chang(National Chiao Tung University) |
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Contributions |
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Infectional efficiency ratio of M13KO7.
Release titer.(Releasing titer of phagemid which is E.coli infected by helper phage)
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Human Practice |
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Eating
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Contributions |
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Held the 2014 Synthetic Biology information meeting of local senior high school and Tzu Chi University as a chief convener.
Contact with National Chiao Tung University for cooperation.
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Wiki |
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House
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Contributions |
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Construct the wiki page.
Coding,coding and coding the wiki page. Study all about Synthetic Biology from wetlab to drylab. |
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And last but not least |
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Video support |
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Wang, Yiru and Chen, Jiawei
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we should be obliged to them for their assistance in this matter. |
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Wang, Yi-ru |
Chen, Jia-wei |
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Special Thanks |
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Our partner : NCTU_Formosa
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We are extremely appreciative of NCTU_Formosa that helped and support us.
The model1 courtesy of Chao-Di Chang.
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Sponsors |
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