Team:BIOSINT Mexico/Modeling

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[[File:Chassis.png|800px|center]]
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<h1>Modeling</h1>
<h1>Modeling</h1>
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<br>This year we designed a construct where a lot of different genes interact in several ways. Eight sets of genes were constructed, using five distinctive kinds of genetic expression. They can be classified under the following categories:
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<br>a) Genes constitutively expressed
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<br>b) Genes that form a protein complex
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<br>c) Auto regulated networks
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<br>d) Simply induced sequences
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<br>e) Complex induced sequences
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'''Equations for Phytochrome-PIF6 complex'''
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<br>In order to produce proteins, in the cell, two main reactions happen. First, DNA is transcripted to mRNA and then it is translated to a peptide sequence, it could be represented in the reaction by:
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<br>
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http://i1379.photobucket.com/albums/ah147/juanjoquispe/modelo/ec1_zpsec1a4694.png
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<br>All these reactions are mediated by two specific enzymes, which are RNA polymerase (transcription) and ribosome (translation). These reactions have been well described in literature and can be modeled to a Ordinary Differential Equation system.
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<br>The rate of production of the PhyB construct is given by the rate of transcription of the protein from the mRNA molecule and the degradation of the messenger. Therefore:
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<br>http://i1379.photobucket.com/albums/ah147/juanjoquispe/modelo/ec2_zpsa7b38e69.png
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Revision as of 17:38, 16 October 2014

Chassis.png

Modeling


This year we designed a construct where a lot of different genes interact in several ways. Eight sets of genes were constructed, using five distinctive kinds of genetic expression. They can be classified under the following categories:
a) Genes constitutively expressed
b) Genes that form a protein complex
c) Auto regulated networks
d) Simply induced sequences
e) Complex induced sequences

Equations for Phytochrome-PIF6 complex
In order to produce proteins, in the cell, two main reactions happen. First, DNA is transcripted to mRNA and then it is translated to a peptide sequence, it could be represented in the reaction by:
http://i1379.photobucket.com/albums/ah147/juanjoquispe/modelo/ec1_zpsec1a4694.png
All these reactions are mediated by two specific enzymes, which are RNA polymerase (transcription) and ribosome (translation). These reactions have been well described in literature and can be modeled to a Ordinary Differential Equation system.
The rate of production of the PhyB construct is given by the rate of transcription of the protein from the mRNA molecule and the degradation of the messenger. Therefore:
http://i1379.photobucket.com/albums/ah147/juanjoquispe/modelo/ec2_zpsa7b38e69.png



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