From 2014.igem.org
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| | <h3>Read about our projects!</h3> | | <h3>Read about our projects!</h3> |
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| - | One E.coli Has One Function
| + | Traditionally pathogens have been annihilated using pharmaceuticals, but today nanotechnology offers a new possibility with the use of nanoelectromechanical systems (NEMS). We present a novel device that could make these processes possible: the BioNEMS Drill. This is a magnetic portion of chromatin. To construct the device we synthesized hydrophilic magnetite. We used it due to its bio-compatibility for medical applications and later functionalized them with amino groups, which can establish a peptide bond between nanoparticles and a protein. We used the HU histone-like protein, capable of coiling DNA around the nanoparticles. To get enough protein, we modified E. Coli to overexpress it. Since the core of the device is magnetic it can be controlled by applying an external magnetic field. Furthermore, the versatility of the DNA to be coated with various materials and its functionalization can selectively remove certain chemicals inside the human body such as cholesterol or toxic agents. |
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| - | What is our project at 2014?
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| - | Cadmium is one of harmful materialｓ for us. 50 years ago, itai-iati disease was going around in center of Japan Gihu. The cause was industrial wastewater. Cadmium contained the water. Our project is to find a way to clean contaminated with Cadmium. we think how to clean the water. We use E.coli.
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| - | Concretely, we use two kinds of E.coli. One catches Cadmium. the Other makes to all E.coli to use chemoattractant. Catches E.coli displays metallothionein (metallothionein is a protein that combines a heavy metal. Cadomium is one kind of heavy metal). Other is releasing Asp (Asp is one kind of chemoattractant. All E.coli is close to the Asp E.coli).
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| - | To use these E.coli. Finally cadomiun is catched(wastewater is be clean). | + | |
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| - | What experiment we did?
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| - | ・How to check E.coli's chemotaxis?
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| - | We make use of other igem team’s assay.
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| - | Using soft agar plate.we let E.coli siwm.
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| - | ・We made a plasmid
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| - | Our project need to two kind of plasmid.
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| - | we made one plasmid.And we have to make one more plasmid.
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Revision as of 17:31, 16 October 2014
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Read about our projects!
Traditionally pathogens have been annihilated using pharmaceuticals, but today nanotechnology offers a new possibility with the use of nanoelectromechanical systems (NEMS). We present a novel device that could make these processes possible: the BioNEMS Drill. This is a magnetic portion of chromatin. To construct the device we synthesized hydrophilic magnetite. We used it due to its bio-compatibility for medical applications and later functionalized them with amino groups, which can establish a peptide bond between nanoparticles and a protein. We used the HU histone-like protein, capable of coiling DNA around the nanoparticles. To get enough protein, we modified E. Coli to overexpress it. Since the core of the device is magnetic it can be controlled by applying an external magnetic field. Furthermore, the versatility of the DNA to be coated with various materials and its functionalization can selectively remove certain chemicals inside the human body such as cholesterol or toxic agents.
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