Team:uOttawa

From 2014.igem.org

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<center> Throughout the life cycle of any given cell, many pre-programmed genetic decisions are made by cells in order for the cells to develop normally or to respond appropriately to external stimuli. This is especially true in complex multi-cellular organisms, where various cell types have to work in tandem in a multitude of cellular tasks. For instance, the apoptotic pathway allows for controlled cell death, which is essential for the continued function of many multicellular organisms. Stem cells must make vital cellular decisions during early development of multicellular organisms about when to differentiate and into what cell type. Thus, it is through understanding the complexities of these ‘decisions’ that scientists may be able to design independent cells that will change its own cellular fate in order to better accomplish whatever its given task. With that in mind, the uOttawa iGEM team is attempting to recreate synthetically one particular cellular decision mechanism within a simply eukaryote (Saccharomyces cerevisiae), taking a step towards controlling entire decision pathways and possibly creating unique cell fates within existing cells.
 
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The mechanism behind pluripotency -- stem cells’ potential to differentiate into all different cell types-- has been a significant focus of modern research. In 2009, Sui Hang et al. theorized that pluripotency is controlled by a cascade of tri-stable switches, a type of genetic network motif that maintains three unique expression states simultaneously.  This year, the uOttawa iGEM team is constructing iGEM’s first functional tri-stable switch based upon this theoretical premise in order to ascertain the potential for tri-stable switches to function as a differentiation mechanism. By doing so, we hope to create genetically engineered cells that are capable of making autonomous cellular decision in reaction to a multitude of internal and external stimuli, allowing us to study this dynamic cellular decision mechanism.</center>
 
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<a href="https://2014.igem.org/Team:uOttawa"style="color:#000000">Home </a> </td>
 
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<a href="https://2014.igem.org/Team:uOttawa/Project"style="color:#000000"> Project</a></td>
 
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<a href="https://2014.igem.org/Team:uOttawa/Modeling"style="color:#000000"> Modeling</a></td>
 
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<a href="https://2014.igem.org/Team:uOttawa/Attributions"style="color:#000000"> Attributions </a></td>
 
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<p> Please be sure to keep these links, your audience will want to find your: </p>
 
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<li><a href="https://2014.igem.org/Team:uOttawa">Home</a> </li>
 
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<li><a href="https://2014.igem.org/Team:uOttawa/Team">Team</a> </li>
 
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=uOttawa">Official Team Profile</a> </li>
 
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<li><a href="https://2014.igem.org/Team:uOttawa/Project">Project</a> </li>
 
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<li><a href="https://2014.igem.org/Team:uOttawa/Modeling">Modeling</a> </li>
 
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<li><a href="https://2014.igem.org/Team:uOttawa/Notebook">Notebook</a> </li>
 
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<li><a href="https://2014.igem.org/Team:uOttawa/Safety">Safety</a> </li>
 
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
 
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
 
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<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
 
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
 
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
 
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
 
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Revision as of 16:01, 16 October 2014

iGEMuOttawa

Take the tour!

How we built the tri-stable switch in S. cerevisae.

Promoters

Eukaryotic, dual-input promoters.

Results

Methods

Measurement

Interlab study

Biobricks

The tri-stable switch

Modelling stem cell differentiation in S. cerevisae.

The tri-stable switch

Modelling stem cell differentiation in S. cerevisae.

Results

Methods

Let's Talk Science

Talking synbio to Ottawa's youth.

The Synthetic Biology course

How to build a new university-level program for synthetic biology at uOttawa.

iGEM in the classroom

Fairs and Festivals

iGEM Mini-Enrichment

oGEM and SynBio Ontario

Genetics and patent law

The hardest-working team in iGEM.

Working hard Hardly working 24/7 since 2008.

Safety

Notebook

Attributions

Retrieved from "http://2014.igem.org/Team:uOttawa"