Team:Toulouse/Notebook/Protocols

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<br>- A microscopy count can be performed thanks to Thoma cell to determine the conidia concentration.
<br>- A microscopy count can be performed thanks to Thoma cell to determine the conidia concentration.
<br>NB : The conidia solutions are then diluted and spread on sap medium to get 10,000 conidia/plate.
<br>NB : The conidia solutions are then diluted and spread on sap medium to get 10,000 conidia/plate.
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<br>- After 72hours of liquid culture of the different clones of <i>B. subtilis>/i> with the fungicides module, the culture can be centrifugated.
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<br>- After 72hours of liquid culture of the different clones of <i>B. subtilis</i> with the fungicides module, the culture can be centrifugated.
<br>- 130µL of the supernatant is used to soak a pad placed on the conidia plate. The bacterial pellet is resuspended in 130µL of LB medium and also put on a pad.  
<br>- 130µL of the supernatant is used to soak a pad placed on the conidia plate. The bacterial pellet is resuspended in 130µL of LB medium and also put on a pad.  
<br>- The plates containing 10,000 conidia and the soaked pads are then put at room temperature for a few days according to the growth speed of the fungi. Controls are also realized with wild type strains or copper sulfate at 10 and 20mg/mL.
<br>- The plates containing 10,000 conidia and the soaked pads are then put at room temperature for a few days according to the growth speed of the fungi. Controls are also realized with wild type strains or copper sulfate at 10 and 20mg/mL.

Revision as of 15:00, 16 October 2014