Team:USyd-Australia/Notebook/Primers

From 2014.igem.org

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<tr><th width="10%">Name</th><th width="50%">Sequence (5'-3')</th><th width="10%">Theoretical Tm</th><th>Purpose<th></tr>
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<tr><td>iGEM10</td><td>AGCTTTCGCTAAGGATGATTTCTGGA</td><td>58.2C</td><td>Screening and sequencing</td></tr>
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<tr><td>iGEM16</td><td>GAGTGCCACCTGACGTCTAAGAAACC</td><td>60.9C</td><td>For BioBrick sequencing, amplification by PCR, or junction PCR.  Alternative to iGEM <a href="http://parts.igem.org/Part:BBa_G00100">VF2</a> primer</td></tr>
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! Name !! Sequence (5'-3') !! Theoretical Tm !! Purpose
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<tr><td>iGEM25</td><td>CCCTGATTCTGTGGATAACCGTATTACCG</td><td>60.0C</td><td>For BioBrick sequencing, amplification by PCR, or junction PCR.  Alternative to iGEM <a href="http://parts.igem.org/Part:BBa_G00101">VR</a> primer</td></tr>
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<tr><td>iGEM1401</td><td>GGGAAGCTTTCTTAGACGTCAGGTGGCACTTTTCGG</td><td>66.3C </td><td> pBBR replicon</td></tr>
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|iGEM10 || AGCTTTCGCTAAGGATGATTTCTGGA || 58.2C || Screening and sequencing
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<tr><td>iGEM1402</td><td>TTTGGATCCTATGCTGCTGGCTACCCTGTGGAAC</td><td>66.5C</td><td>pBBR replicon</td></tr>\
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<tr><td>iGEM1403</td><td>GTTCCACAGGGTAGCCAGCAGCATAGGATCCAAAGAGTGCCACCTGACGTCTAAGAAACC</td><td>71.2C</td><td>primers for pSB prefix/suffix</td></tr>
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|iGEM16 || GAGTGCCACCTGACGTCTAAGAAACC ||  60.9C || For BioBrick sequencing, amplification by PCR, or junction PCR.  Alternative to iGEM [http://parts.igem.org/Part:BBa_G00100 VF2] primer
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<tr><td>iGEM1404</td><td>CCGAAAAGTGCCACCTGACGTCTAAGAAAGCTTCCCCCTGATTCTGTGGATAACCGTATTACCG</td><td>70.3</td><td>primers for pSB prefix/suffix</td></tr>
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<tr><td>iGEM1405</td><td>GAGGTCGGACAGTATATTGAAACGTCAGGAGTCTAGATACCGAGGGACAGACTACCAACTCACA</td><td> </td><td>primers for gene cassette circularisation (attC-aeBlue-aacC1)</td></tr>
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|iGEM25 || CCCTGATTCTGTGGATAACCGTATTACCG ||  60.0C || For BioBrick sequencing, amplification by PCR, or junction PCR.  Alternative to iGEM [http://parts.igem.org/Part:BBa_G00101 VR] primer
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<tr><td>iGEM1406</td><td> GTATCTAGACTCCTGACGTTTCAATATACTGTCCGACCTCTTATTAGGTGGCGGTACTTGGGTCG</td><td> </td><td>primers for gene cassette circularisation (attC-aeBlue-aacC1)</td></tr>
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<tr><td>iGEM1407 </td><td>CTATATCTATGATCTCGCAGTCTCC </td><td>53.8C </td><td> PCR primers over the junction between aacC1 (G block) and pSB1C3 vector, for validation of successful G block insertion by PCR screening</td></tr>
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|iGEM1401 || GGGAAGCTTTCTTAGACGTCAGGTGGCACTTTTCGG || 66.3C || pBBR replicon
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|iGEM1402 || TTTGGATCCTATGCTGCTGGCTACCCTGTGGAAC || 66.5C || pBBR replicon
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|iGEM1403 || GTTCCACAGGGTAGCCAGCAGCATAGGATCCAAAGAGTGCCACCTGACGTCTAAGAAACC || 71.2C || primers for pSB prefix/suffix
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|iGEM1404 || CCGAAAAGTGCCACCTGACGTCTAAGAAAGCTTCCCCCTGATTCTGTGGATAACCGTATTACCG || 70.3 || primers for pSB prefix/suffix
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|iGEM1405 || GAGGTCGGACAGTATATTGAAACGTCAGGAGTCTAGATACCGAGGGACAGACTACCAACTCACA || || primers for gene cassette circularisation (attC-aeBlue-aacC1)
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|iGEM1406 || GTATCTAGACTCCTGACGTTTCAATATACTGTCCGACCTCTTATTAGGTGGCGGTACTTGGGTCG || || primers for gene cassette circularisation (attC-aeBlue-aacC1)
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|iGEM1407 || CTATATCTATGATCTCGCAGTCTCC || 53.8C || PCR primers over the junction between aacC1 (G block) and pSB1C3 vector, for validation of successful G block insertion by PCR screening
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|iGEM1408 || GCCTTTTGCTCACATGTTCTTTC || 55.2C || PCR primers over the junction between aacC1 (G block) and pSB1C3 vector, for validation of successful G block insertion by PCR screening
|iGEM1408 || GCCTTTTGCTCACATGTTCTTTC || 55.2C || PCR primers over the junction between aacC1 (G block) and pSB1C3 vector, for validation of successful G block insertion by PCR screening
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|NVC159 || GCCGCCTTGGGCCGGGTGATGTC || 69.2C || pUS41/44, right of cloning site
|NVC159 || GCCGCCTTGGGCCGGGTGATGTC || 69.2C || pUS41/44, right of cloning site
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{{Team:USyd-Australia/Footer}}
{{Team:USyd-Australia/Footer}}

Revision as of 14:45, 16 October 2014

iGEM_Link


Primers

\ |- |iGEM1408 || GCCTTTTGCTCACATGTTCTTTC || 55.2C || PCR primers over the junction between aacC1 (G block) and pSB1C3 vector, for validation of successful G block insertion by PCR screening |- |iGEM1409 || GTTTTTTTGGATGGAGTGAAACGATGGCGATTG || 61.7C || Amplification of iGEM-IntI1 gBlock forward primer |- |iGEM1410 || TGACACCTTGCCCTTTTTTGCCG || 60.9C || Amplification of iGEM-IntI1 gBlock reverse primer |- |iGEM1411 || GTTTTTTTGGATGGAGTGAAACGATGGCGATTG || 61.7C || Gblock primers for IntI1 in pSAM-R |- |iGEM1412 || TGACACCTTGCCCTTTTTTG || 53.9C || Gblock primers for IntI1 in pSAM-R |- |iGEM1413 || GACATTGCCGTCACTGCGTC || 59.1C || Junction primers for IntI1 in pSAM-R |- |iGEM1414 || TGGTCCAGAACCTTGACCGAAC || 59.2C || Junction primers for IntI1 in pSAM-R |- |iGEM1415 || AACCGAGGATGCGAACCACTTC || 59.7C || Junction primers for IntI1 in pSAM-R |- |iGEM1416 || CCTTCAAACGTGCCGTAGAACAAGC || 60.4C || Junction primers for IntI1 in pSAM-R |- |iGEM1417 || AAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAG || 67.6C || Linearisation of pSB1C3, containing full BioBrick prefix and suffix |- |iGEM1418 || AAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGC || 66.9C || Linearisation of pSB1C3, containing full BioBrick prefix and suffix |- |iGEM1419 || TTTGAATTCGCGGCCGCTTCTAGAGTACCGAGGGACAGAC || 68.9C || Amplification of aeBlue gBlock. Forward primer. |- |iGEM1420 || GGGCTGCAGCGGCCGCTACTAGTATTATTAGGTGG || 67.4C || Amplification of aeBlue gBlock. Reverse primer. |- |iGEM1421 || GGGAATTCAAATCTAGAGACACCATCG || 57.1C || Amplification of LacI-Plac-AttI gBlock. Forward primer |- |iGEM1422 || CCTGCAGTTTACTAGTTTTGCCTAACTTTGTTTTAG || 59.4C || Amplification of LacI-Plac-AttI gBlock. Reverse primer |- |iGEM1423 || CAGGCTTTACACTTTATGCTTCCG || 56.3C || lacI-Plac-attI RHJ-F right hand junction forward primer (use with iGEM25) |- |iGEM1424 || AATGTAATTCAGCTCCGCCATC|| 55.5C || lacI-Plac-attI LHJ-R left hand junction reverse primer (use with iGEM16) |- |iGEM1425 || AAATCTAGATGGCGCGGCTTAACTCAGGTGTTAGGCTTAGGAGGCTCAAGTATGGGCAT || 70.7C || For making aacC1 gene cassettes from Tn1696. Use with iGEM1426. |- |iGEM1426 || AAAACTAGTGGCGTCGGCTTGGACGAATTGTTAGGCTTAGGTGGCGGTACTTGGGTC || 71.4C || For making aacC1 gene cassettes from Tn1696. Use with iGEM1425. |- |iGEM1427 || TTTTCTAGAGTTTGATGTTATGGAGCAGCAACG || 60.2C || For making aacC1 gene cassettes from Tn1696. Use with iGEM1428. Alternative to iGEM1424. |- |iGEM1428 || TTTACTAGTGCAAAAAGGCAGCAATTATGAGCC || 60.9C || For making aacC1 gene cassettes from Tn1696. Use with iGEM1427. Alternative to iGEM1426. |- |NVC71 || CTAAGAATCCATAGTCCAACTCC || 52.4C || aadB gene, rvs primer for junction screen |- |NVC92b || CACGCAAGACCTCAACCTTTTCC || 58.6C || aadB gene, rvs primer for junction screen |- |NVC158 || GATACCTTGTGCGGCTATGTCTG || 57.6C || pUS41/44, left of cloning site |- |NVC159 || GCCGCCTTGGGCCGGGTGATGTC || 69.2C || pUS41/44, right of cloning site |}
NameSequence (5'-3')Theoretical TmPurpose
iGEM10AGCTTTCGCTAAGGATGATTTCTGGA58.2CScreening and sequencing
iGEM16GAGTGCCACCTGACGTCTAAGAAACC60.9CFor BioBrick sequencing, amplification by PCR, or junction PCR. Alternative to iGEM VF2 primer
iGEM25CCCTGATTCTGTGGATAACCGTATTACCG60.0CFor BioBrick sequencing, amplification by PCR, or junction PCR. Alternative to iGEM VR primer
iGEM1401GGGAAGCTTTCTTAGACGTCAGGTGGCACTTTTCGG66.3C pBBR replicon
iGEM1402TTTGGATCCTATGCTGCTGGCTACCCTGTGGAAC66.5CpBBR replicon
iGEM1403GTTCCACAGGGTAGCCAGCAGCATAGGATCCAAAGAGTGCCACCTGACGTCTAAGAAACC71.2Cprimers for pSB prefix/suffix
iGEM1404CCGAAAAGTGCCACCTGACGTCTAAGAAAGCTTCCCCCTGATTCTGTGGATAACCGTATTACCG70.3primers for pSB prefix/suffix
iGEM1405GAGGTCGGACAGTATATTGAAACGTCAGGAGTCTAGATACCGAGGGACAGACTACCAACTCACA primers for gene cassette circularisation (attC-aeBlue-aacC1)
iGEM1406 GTATCTAGACTCCTGACGTTTCAATATACTGTCCGACCTCTTATTAGGTGGCGGTACTTGGGTCG primers for gene cassette circularisation (attC-aeBlue-aacC1)
iGEM1407 CTATATCTATGATCTCGCAGTCTCC 53.8C PCR primers over the junction between aacC1 (G block) and pSB1C3 vector, for validation of successful G block insertion by PCR screening

With thanks to:

Retrieved from "http://2014.igem.org/Team:USyd-Australia/Notebook/Primers"