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Team:UFAM Brazil/Protocol8
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Revision as of 14:21, 16 October 2014
Ligation | ||
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Steps: 1. Add 2ul of digested plasmid backbone (25 ng) 2. Add equimolar amount of digested fragment (< 3 µl) 3. Add 1 ul 10X T4 DNA ligase buffer. 4. Add 0.5 ul T4 DNA ligase 5. Add water to 10 ul. 6. Ligation 16 °C over night. 7. Transform with 1-2 ul of product. In the case of low concentration of DNA ligated, purify and concentrate the ligation system. | ||
| Methods | ||
