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Team:Groningen/Template/MODULE/PP/collaborations/methods
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| - | We I performed a halo experiment to test if S. aureus has sensitivity to subtilin, produced by B. subtilis. This was performed in the ML-II lab of the MolGen group at the University of Groningen and supervised by Renske van Raaphorst. Below we report what steps are taken and give an overview of the results. Also we provide you with some references of the strains we used. | + | We I performed a halo experiment to test if <i>S. aureus</i> has sensitivity to subtilin, produced by <i>B. subtilis</i>. This was performed in the ML-II lab of the MolGen group at the University of Groningen and supervised by Renske van Raaphorst. Below we report what steps are taken and give an overview of the results. Also we provide you with some references of the strains we used. |
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Revision as of 13:36, 16 October 2014
Methods
We I performed a halo experiment to test if S. aureus has sensitivity to subtilin, produced by B. subtilis. This was performed in the ML-II lab of the MolGen group at the University of Groningen and supervised by Renske van Raaphorst. Below we report what steps are taken and give an overview of the results. Also we provide you with some references of the strains we used.
30-09-2014
- Inoculated three different strains of ''S. aureus'' (see below) in 3 ml Nutrient Broth (Difco). Also two different ''B. subtilis'' strains were inoculated under different conditions (see below). The ATCC6633 strain was inoculated in 3 ml LB and in 3 ml of the defined medium SMM. Also the 168 strain was inoculated in 3 ml LB. These samples were grown overnight at 37ºC in a shaking incubator.
I'AM A BULLET COPY ME AND EXPLAIN ME.
