Team:Tianjin/Human Practice
From 2014.igem.org
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<td bgcolor="#FFED97"><img width="241" height="187" src="https://static.igem.org/mediawiki/2014/f/f1/Human-practice2_clip_image002_0001.gif" /></td> | <td bgcolor="#FFED97"><img width="241" height="187" src="https://static.igem.org/mediawiki/2014/f/f1/Human-practice2_clip_image002_0001.gif" /></td> | ||
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<p class="STYLE33"> </p> | <p class="STYLE33"> </p> | ||
+ | <table width="100%" border="0" cellspacing="0" cellpadding="0"> | ||
+ | <tr> | ||
+ | <td><table width="83%" border="1" align="center" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
+ | <tr> | ||
+ | <td width="210" valign="top"><p>M</p></td> | ||
+ | <td width="357" valign="top"><p>The concentration of total MinC proteins</p></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="210" valign="top"><p>N</p></td> | ||
+ | <td width="357" valign="top"><p>Cell density</p></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="210" valign="top"><p>R</p></td> | ||
+ | <td width="357" valign="top"><p>The concentration of total proteins[LuxR] </p></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="210" valign="top"><p>A</p></td> | ||
+ | <td width="357" valign="top"><p>The number of AHL signal</p></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="210" valign="top"><p>k</p></td> | ||
+ | <td width="357" valign="top"><p>Growth constant</p></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="210" valign="top"><p>kA</p></td> | ||
+ | <td width="357" valign="top"><p>The binding rate between AHL and LuxR proteins</p></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="210" valign="top"><p>kM</p></td> | ||
+ | <td width="357" valign="top"><p>Production rate of MinC.</p></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="210" valign="top"><p>kR </p></td> | ||
+ | <td width="357" valign="top"><p>The production rate of LuxR proteins</p></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="210" valign="top"><p>d</p></td> | ||
+ | <td width="357" valign="top"><p>The death rate related with MinC proteins.</p></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td width="210" valign="top"><p>di</p></td> | ||
+ | <td width="357" valign="top"><p>The degradation rate of materials.</p></td> | ||
+ | </tr> | ||
+ | </table></td> | ||
+ | </tr> | ||
+ | </table> | ||
<p class="STYLE33"> Then we use these equations to simulate the function of MIN system. The results are shown as figure 1 and figure 2.</p> | <p class="STYLE33"> Then we use these equations to simulate the function of MIN system. The results are shown as figure 1 and figure 2.</p> | ||
<table width="100%" border="0" cellpadding="0" cellspacing="0" bordercolor="#FFED97" bgcolor="#FFED97"> | <table width="100%" border="0" cellpadding="0" cellspacing="0" bordercolor="#FFED97" bgcolor="#FFED97"> | ||
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- | <td bgcolor="#FFED97"><img width="350" height=" | + | <td bgcolor="#FFED97"><img width="350" height="250" src="https://static.igem.org/mediawiki/2014/d/dd/Tianjin-hp-o2.png" /></td> |
- | <td bgcolor="#FFED97"><img width="350" height=" | + | <td bgcolor="#FFED97"> </td> |
+ | <td bgcolor="#FFED97"><img width="350" height="250" src="https://static.igem.org/mediawiki/2014/4/4b/Tianjin-hp-o3.png" /></td> | ||
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- | <td bgcolor="#FFED97"><strong>Figure 1</strong>. The dynamic change of cell number and total number of LuxR proteins. After a sharp increase, the cell stopped multiplying themselves. With the growing of E.coli, the total number of LuxR proteins also increases. The initial value of cell number we set is 3000.</td> | + | <td bgcolor="#FFED97" class="STYLE33"><strong>Figure 1</strong>. The dynamic change of cell number and total number of LuxR proteins. After a sharp increase, the cell stopped multiplying themselves. With the growing of E.coli, the total number of LuxR proteins also increases. The initial value of cell number we set is 3000.</td> |
- | <td bgcolor="#FFED97"><strong>Figure 2.</strong> The dynamic change of MinC proteins and AHL signal. The AHL decrease very quickly with the growth of E.coli. And when AHL comes to the threshold value, the repression of MinC is removed. So after some time the MinC proteins increase quickly and the cell division process is inhibited.</td> | + | <td bgcolor="#FFED97"> </td> |
+ | <td bgcolor="#FFED97" class="STYLE33"><strong>Figure 2.</strong> The dynamic change of MinC proteins and AHL signal. The AHL decrease very quickly with the growth of E.coli. And when AHL comes to the threshold value, the repression of MinC is removed. So after some time the MinC proteins increase quickly and the cell division process is inhibited.</td> | ||
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- | <td bgcolor="#FFED97"><img src="https://static.igem.org/mediawiki/2014/ | + | <td bgcolor="#FFED97"><img src="https://static.igem.org/mediawiki/2014/b/b9/Tianjin-hp-o4.png" alt="Screen Shot 2014-10-16 at 4" width="432" height="300" align="middle" /></td> |
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- | <td bgcolor="#FFED97"><strong>Figure 3.</strong>Cell growth curve of BBa_J23100 and parallel that a relatively weaker promoter was used to control the CsgA secrete level</td> | + | <td bgcolor="#FFED97" class="STYLE33"><strong>Figure 3.</strong>Cell growth curve of BBa_J23100 and parallel that a relatively weaker promoter was used to control the CsgA secrete level</td> |
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- | <td bgcolor="#FFED97"><img width="540" height=" | + | <td bgcolor="#FFED97"><img width="540" height="400" src="https://static.igem.org/mediawiki/2014/6/6d/Tianjin-hp-o5.png" align="middle" hspace="12" alt="Screen Shot 2014-10-16 at 4" /></td> |
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- | <td bgcolor="#FFED97"><strong>Figure 4. </strong>Absorbency of secrete protein in culture between K1361007 and parallel. No significant divergence was found.</td> | + | <td bgcolor="#FFED97" class="STYLE33"><strong>Figure 4. </strong>Absorbency of secrete protein in culture between K1361007 and parallel. No significant divergence was found.</td> |
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Revision as of 12:46, 16 October 2014
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