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Team:Goettingen/proLP
From 2014.igem.org
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<li><a href="https://2014.igem.org/Team:Goettingen/protocol_DNA#rest_DNA">Restriction of DNA</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_DNA#rest_DNA">Restriction of DNA</a></li> | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_DNA#liga_DNA">Ligation of DNA fragments</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_DNA#liga_DNA">Ligation of DNA fragments</a></li> | ||
| - | + | ||
</ul> | </ul> | ||
<h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con">Plasmid Construction</a></h4> | <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con">Plasmid Construction</a></h4> | ||
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<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#BP">BP recombination reaction</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#BP">BP recombination reaction</a></li> | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#LR">LR recombination reaction</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#LR">LR recombination reaction</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#seam_clone">SEAMLESS Cloning</a></li> | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#LB">Peptide Library construction</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Plasmid_Con#LB">Peptide Library construction</a></li> | ||
</ul> | </ul> | ||
Revision as of 11:58, 16 October 2014
Overview
PCR Methods
DNA Assessment
Plasmid Construction
Plasmid Transformation
- E.coli competent cells
- Plasmid isolation (E.coli)
- E.coil transformation
- Plasmid isolation (Yeast)
- Yeast transformation
