Team:NJU-QIBEBT/humanPractice

From 2014.igem.org

(Difference between revisions)
Line 378: Line 378:
         A club named iGEM was formed by our team and another team ’Nanjing-China’ in our campus. We can publicize the idea of the iGEM competition to other students and thus it can attract more students to join this competition. Furthermore, this club intends to recruit some members from other departments and our team can therefore be more versatile. Anyway, the establishment of this club will enable the iGEM competition to continue at our university in the future.
         A club named iGEM was formed by our team and another team ’Nanjing-China’ in our campus. We can publicize the idea of the iGEM competition to other students and thus it can attract more students to join this competition. Furthermore, this club intends to recruit some members from other departments and our team can therefore be more versatile. Anyway, the establishment of this club will enable the iGEM competition to continue at our university in the future.
     </p>
     </p>
-
</div>
 
-
 
-
{{Team:NJU-QIBEBT/CSS/Reset}}
 
-
<html>
 
-
<head>
 
-
<title></title>
 
-
<meta charset="utf-8">
 
-
<script src="http://libs.baidu.com/jquery/1.8.1/jquery.min.js"></script>
 
-
<script type="text/javascript">
 
-
    $(function(){
 
-
        $(".button").click(function{
 
-
           
 
-
        })
 
-
        $(window).scroll(function(){
 
-
            var sTop = Math.max(document.body.scrollTop || document.documentElement.scrollTop);
 
-
 
-
            if(sTop > 260 ){
 
-
                $("#fixedMenu").css({'position':'fixed','top':'0'});
 
-
                $("#fixedMenu_2").css({
 
-
                    'display':'block'
 
-
                });
 
-
                $("#top-section").css({
 
-
                    'display':'none'
 
-
                });
 
-
 
-
            }
 
-
            else{
 
-
                $("#fixedMenu").css({'position':'relative'})
 
-
                $("#fixedMenu_2").css({
 
-
                    'display':'none'
 
-
                });
 
-
 
-
            }
 
-
        })
 
-
    })
 
-
</script>
 
-
 
-
<link href='http://fonts.useso.com/css?family=PT+Sans:400,700,400italic' rel='stylesheet' type='text/css'>
 
-
<style type="text/css">
 
-
    *{
 
-
        padding:0;
 
-
        margin: 0;
 
-
    }
 
-
    a{
 
-
        text-decoration: none;
 
-
        color: #333333;
 
-
    }
 
-
 
-
    #header{
 
-
        margin: 0 auto;
 
-
        width: 100%;
 
-
    }
 
-
    /*========================*/
 
-
    #nav-bottom {
 
-
        width: 100%;
 
-
        height: 50px;
 
-
        background:url("https://static.igem.org/mediawiki/2014/a/ae/10.jpg");
 
-
    }
 
-
    ul#nav_content{
 
-
        width: 960px;
 
-
        margin: 0 auto;
 
-
        padding: 0;
 
-
        list-style: none;
 
-
 
-
    }
 
-
    ul#nav_content a{
 
-
        display: inline-block;
 
-
        line-height: 50px;
 
-
        color: #333333;
 
-
        padding: 0 12px;
 
-
        transition:all 0.4s;
 
-
        font-size: 18px;
 
-
        font-family: 'PT Sans','Microsoft Yahei','Georgia' ,sans-serif;
 
-
 
-
 
-
 
-
    }
 
-
    ul#nav_content a:hover{
 
-
        background-color: rgba(51,51,51,0.4);
 
-
        color: whitesmoke;
 
-
 
-
    }
 
-
    ul#nav_content ul{
 
-
        padding: 0;
 
-
        list-style: none;
 
-
        float: left;
 
-
        margin-top: 0px;
 
-
        margin-left: 0px;
 
-
 
-
    }
 
-
    ul#nav_content li{
 
-
        float: left;
 
-
    }
 
-
    ul#nav_content li ul li{
 
-
        background-color: rgba(51,51,51,0.3);
 
-
        float: left;
 
-
        border-radius: 3px;
 
-
        transition:all 0.4s;
 
-
 
-
    }
 
-
    ul#nav_content li ul li{
 
-
    }
 
-
    ul#nav_content li ul{
 
-
        position: absolute;
 
-
        left: -999em;
 
-
 
-
    }
 
-
    #nav_content li:hover ul{
 
-
        left: auto;
 
-
    }
 
-
</style>
 
-
 
-
<style type="text/css">
 
-
    #top-section {
 
-
        background:rgba(0,0,0,0.1);}
 
-
    .firstHeading{
 
-
        display:none; }
 
-
    li{
 
-
        list-style: none;
 
-
    }
 
-
    *{
 
-
        padding:0;
 
-
        margin: 0;
 
-
    }
 
-
    a{
 
-
        text-decoration: none;
 
-
        color: #333333;
 
-
    }
 
-
    body{
 
-
        background: url("https://static.igem.org/mediawiki/2014/3/30/NJU_Bac.jpg") fixed;
 
-
 
-
        padding:0;
 
-
        margin: 0;
 
-
    }
 
-
    }
 
-
    #header{
 
-
        margin: 0 auto;
 
-
        width: 100%;
 
-
    }
 
-
    #nav-top{
 
-
        width: 100%;
 
-
        margin: 0 auto;
 
-
        height: 100%;
 
-
 
-
    }
 
-
    /*
 
-
    #nav-bottom a{
 
-
        text-align: center;
 
-
        top: 14px;
 
-
        position: relative;
 
-
        display: inline-block;
 
-
        font-size: 18px;
 
-
        height: 54px;
 
-
        padding-left: 10px;
 
-
        padding-right: 10px;
 
-
        margin: 0;
 
-
        color: #e7e7e7;
 
-
        text-shadow: 2px 2px 3px #333333;
 
-
    }
 
-
    */
 
-
    #back_to_top {
 
-
        position: fixed;
 
-
        left: 50%;
 
-
        margin-left: 500px;
 
-
        width: 100px;
 
-
        height: 100px;
 
-
        bottom: 30px;
 
-
        background: url("https://static.igem.org/mediawiki/2014/2/2e/Nju_back_to_top.png");
 
-
    }
 
-
    #back_to_top:hover{
 
-
        background: url("https://static.igem.org/mediawiki/2014/3/35/Nju_back_to_top.gif");
 
-
    }
 
-
    #footer{
 
-
        width: 100%;
 
-
        background: url("https://static.igem.org/mediawiki/2014/6/6f/NJU_motif_grille.png") repeat;
 
-
        height: 200px;
 
-
 
-
    }
 
-
    #footer img{
 
-
        height: 75px;
 
-
        margin-top: 10px;
 
-
    }
 
-
    #footer #footerContent{
 
-
        width: 960px;
 
-
        overflow: hidden;
 
-
        color: #FAFAFA;
 
-
        margin: 0 auto;
 
-
    }
 
-
    #footer #footerContent #footerLeft{
 
-
        width: 400px;
 
-
        float: left;
 
-
        margin-top: 10px;
 
-
    }
 
-
    #footer #footerContent #footerRight{
 
-
        width: 500px;
 
-
        float: right;
 
-
        margin-top: 10px;
 
-
    }
 
-
    #footer #footerContent p{
 
-
        padding-top: 5px;
 
-
        font-family: 'PT Sans','Microsoft Yahei','Georgia' ,sans-serif;
 
-
    }
 
-
    #footer #footerContent h3{
 
-
        font-size: 1.5em;
 
-
        padding-top: 5px;
 
-
        font-family: 'PT Sans','Microsoft Yahei','Georgia' ,sans-serif;
 
-
        font-weight: 700;
 
-
    }
 
-
    #nav-bottom {
 
-
        width: 100%;
 
-
        height: 50px;
 
-
        background:url("https://static.igem.org/mediawiki/2014/a/ae/10.jpg");
 
-
    }
 
-
    #main{
 
-
        z-index: 99;
 
-
        padding: 10px;
 
-
        background: url("https://static.igem.org/mediawiki/2014/f/f3/Halftone.png") repeat;
 
-
    }
 
-
</style>
 
-
<style type="text/css">
 
-
        a.button{
 
-
        width: 70px;
 
-
        height: 80px;
 
-
        background:url("https://static.igem.org/mediawiki/2014/0/0e/Light_on.png");
 
-
        background-size: 70px;
 
-
        z-index: 99;
 
-
        position: absolute;
 
-
        left: 80%;
 
-
        top: 5%;
 
-
    }
 
-
    #header{
 
-
        margin-bottom: 10px;
 
-
    }
 
-
    #main{
 
-
        width: 960px;
 
-
        margin: 0 auto;
 
-
        box-shadow: 0px 1px 5px #111111;
 
-
 
-
    }
 
-
    #main #fixedMenu{
 
-
        padding:10px;
 
-
        font-family: 'PT Sans','Microsoft Yahei','Georgia' ,sans-serif;
 
-
        font-size: 2.8em;
 
-
        display: inline-block;
 
-
        color: #e7e7e7;
 
-
        background-color: rgba(51,51,51,0.5);
 
-
        width: 200px;
 
-
        padding-left: 10px;
 
-
 
-
    }
 
-
    #main p{
 
-
        font-family: 'PT Sans','Microsoft Yahei','Georgia' ,sans-serif;
 
-
    }
 
-
    #fixedMenu_2{
 
-
        display: none;
 
-
        height: 36px;
 
-
    }
 
-
    #sideMenu{
 
-
        position: fixed;
 
-
        display: none;
 
-
    }
 
-
</style>
 
-
<style type="text/css">
 
-
    #main p{
 
-
        margin-top: 10px;
 
-
        margin-bottom: 10px;
 
-
        font-size: 18px;
 
-
    }
 
-
    #main img{
 
-
        margin-left: 20px;
 
-
        margin-top: 30px;
 
-
        margin-bottom: 30px;
 
-
        margin-left: 30px;
 
-
    }
 
-
    #main h1{
 
-
        font-size: 36px;
 
-
        font-family:'PT Sans','Microsoft Yahei','Georgia' ,sans-serif;
 
-
        font-weight: 700;
 
-
    }
 
-
    #main h2{
 
-
        font-size: 28px;
 
-
        font-weight: 400;
 
-
        font-family:'PT Sans','Microsoft Yahei','Georgia' ,sans-serif;
 
-
        margin-top: 10px;
 
-
    }
 
-
    #main h3{
 
-
        font-family:'PT Sans','Microsoft Yahei','Georgia' ,sans-serif;
 
-
        font-style: italic;
 
-
    }
 
-
    #main h1,#main h2,#main h3{
 
-
        border-bottom:none;
 
-
    }
 
-
    .se_title{
 
-
        font-family:'PT Sans','Microsoft Yahei','Georgia' ,sans-serif;
 
-
        font-size: 18px;
 
-
        font-weight: 700;
 
-
    }
 
-
    .se_title_talic{
 
-
        font-style: italic;
 
-
        color: #6166cc;
 
-
        font-size: 18px;
 
-
    }
 
-
 
-
</style>
 
-
</head>
 
-
<body>
 
-
<div id="header">
 
-
    <div id="nav-top">
 
-
        <img src="https://static.igem.org/mediawiki/2014/7/75/Njuday_%281%29-1.jpg" width="100%">
 
-
        <a href="javascript:" class="button"></a>
 
-
    </div>
 
-
    <div id="nav-bottom">
 
-
        <ul id="nav_content">
 
-
            <li class="menu"><a href="/Team:NJU-QIBEBT">HOME</a>
 
-
            </li>
 
-
            <li class="menu"><a href="background">PROJECT</a>
 
-
                <ul>
 
-
                    <li><a href="/Team:NJU-QIBEBT/team/Overview">Overview</a></li>
 
-
                    <li><a href="/Team:NJU-QIBEBT/team/Backgroud">Backgroud</a></li>
 
-
                    <li><a href="/Team:NJU-QIBEBT/Production module">Production module</a></li>
 
-
                    <li><a href="/Team:NJU-QIBEBT/Pumping out module">Pumping out module</a></li>
 
-
                    <li><a href="/Team:NJU-QIBEBT/Indicator module">Indicator module</a></li>
 
-
                </ul>
 
-
 
-
            </li>
 
-
            <li class="menu"><a href="/Team:NJU-QIBEBT/ACHIEVEMENT">ACHIEVEMENT</a>
 
-
                <ul>
 
-
                    <li><a href="/Team:NJU-QIBEBT/ACHIEVEMENT/Parts test results">Parts test results</a></li>
 
-
                    <li><a href="/Team:NJU-QIBEBT/ACHIEVEMENT/Modeling">Modeling</a></li>
 
-
                    <li><a href="/Team:NJU-QIBEBT/ACHIEVEMENT/Judging Criteria">Judging Criteria</a></li>
 
-
                </ul>
 
-
            </li>
 
-
            <li class="menu"><a href="/Team:NJU-QIBEBT/wetlab">WET LAB</a>
 
-
                <ul>
 
-
                    <li><a href="/Team:NJU-QIBEBT/wetlab/Protocol">Protocol</a></li>
 
-
                    <li><a href="/Team:NJU-QIBEBT/wetlab/Notebook">Notebook</a></li>
 
-
                    <li><a href="/Team:NJU-QIBEBT/wetlab/Parts">Parts</a></li>
 
-
                </ul>
 
-
 
-
            </li>
 
-
            <li class="menu"><a href="/Team:NJU-QIBEBT/humanPractice">HUMAN PRACTICE</a></li>
 
-
            <li class="menu"><a href="/Team:NJU-QIBEBT/SAFETY">SAFETY</a></li>
 
-
            <li class="menu"><a href="/Team:NJU-QIBEBT/team">TEAM</a></li>
 
-
            <li class="menu"><a href="/Team:NJU-QIBEBT/ACKNOWLEDGEMENT">ACKNOWLEDGEMENT</a></li>
 
-
        </ul>
 
-
        <!--
 
-
        <div id="content1" style="width: 720px;margin: 0 auto">
 
-
        <a href="/Team:NJU-QIBEBT/Extras" >Extras<img src="img/nav_r.png"></a><a href="/Team:NJU-QIBEBT/Safety">Safety</a><a href="/Team:NJU-QIBEBT/Modeling">Modeling</a><a href="/Team:NJU-QIBEBT/Practice" >Practice<img src="img/nav_r.png"></a><a href="/Team:NJU-QIBEBT/Parts">Parts</a><a href="/Team:NJU-QIBEBT/Team">Team</a><a href="/Team:NJU-QIBEBT/Project">Project</a><a href="/Team:NJU-QIBEBT">Home</a>
 
-
        </div>
 
-
        -->
 
-
    </div>
 
-
 
-
</div>
 
-
<div id="sideMenu" style="display: none">
 
-
    <ul>
 
-
        <li><a>111111</a></li>
 
-
        <li><a>222222</a></li>
 
-
        <li><a>333333</a></li>
 
-
        <li><a>444444</a></li>
 
-
    </ul>
 
-
</div>
 
-
<div id="main" style="min-height:1000px">
 
-
    <h1>Attribution</h1>
 
-
    <h2>Project</h2>
 
-
    <h3>Production increase and control module
 
-
    </h3>
 
-
    <p>Isolation of promoter and operator sequences from lac operon and ara operon: Xu Chunhui, Wen Yu Qi, Zhang Qiuhao and Zhao Yiling
 
-
    </p>
 
-
    <p>Isolation of promoter and operator sequences from trp operon: Xu Yilin, Chen Yuda, Yang Zhanzhao
 
-
    </p>
 
-
    <p>Plasmid construction with lac operon, ara operon, AtfatA and bte: Qin Zhen, Rao Bing, Rong Hui, Zhou Yu and Zhu Yi
 
-
    </p>
 
-
    <p>Plasmid construction with trp operon, fadA and fadB: Xu Yilin, Chen Yuda, Yang Zhanzhao and Zhu Qizhang
 
-
    </p>
 
-
    <p>QPCR: Zhu Yi, Qin Zhen, Zhou Yu, Xu Yilin
 
-
    </p>
 
-
    <p>Plasmid function test in QIEBET: Rao Bing, Zhu Ruichi and Zhou Yu
 
-
    </p>
 
-
    <p>Advisor: Liu Yanqing, Wang Xueliang
 
-
    </p>
 
-
    <h3>Pumping out module
 
-
    </h3>
 
-
    <p>Plasmid construction with msbA and pfadD: Qin Zhen, Rao Bing, Rong Hui, Zhu Yi and Zhu Ruichi
 
-
    </p>
 
-
    <p>QPCR: Rao Bing, Rong Hui
 
-
    </p>
 
-
    <p>Plasmid function test in QIEBET: Rao Bing, Zhu Ruichi and Zhou Yu
 
-
    </p>
 
-
    <p>Advisor: Liu Yanqing, Wang Xueliang
 
-
    </p>
 
-
    <h3>Indicator module
 
-
    </h3>
 
-
    <p>Plasmid construction: Ou Yangli
 
-
    </p>
 
-
    <p>Advisor: Xiong Aoli
 
-
    </p>
 
-
    <p>Plasmid function test: Rao Bing, Zhu Ruichi and Zhou Yu
 
-
    </p>
 
-
    <p>QPCR: Ou Yangli, Zhang Qiuhao, Xu Chunhui
 
-
    </p>
 
-
    <h2>Modeling</h2>
 
-
    <p>Data analysis: Ou Yangli, Zhao Yiling,
 
-
    </p>
 
-
    <p>Construction: Bu Jinzhi
 
-
    </p>
 
-
    <h2>Painting</h2>
 
-
    <p>Xu Yilin, Qin zhen
 
-
    </p>
 
-
    <h2>Wiki</h2>
 
-
    <p class="se_title">Designer:
 
-
    </p>
 
-
    <p>Parts, protocol and notebook collection: Xu Chunhui, Zhang Qiuhao, Yang Zhanzhao, Chen Yuda, Zhu Qizhang, Zhao Yiling, Wen Yuqi and Ou Yangli
 
-
    </p>
 
-
    <p>Editor: Zhu Yi, Zhu Ruichi, Rong Hui, Zhang Qiuhao
 
-
    </p>
 
-
    <p>Subeditor: other team members
 
-
    </p>
 
-
    <h2>Human Practice    </h2>
 
-
    <p>Meetup in Wu Han: Qin Zhen, Rong Hui, Zhang Qiuhao and Zhou Yu
 
-
    </p>
 
-
    <p>Internship in QIBEBT: Rao Bing, Zhu Ruichi and Zhou Yu
 
-
    </p>
 
-
    <p>Small-scale industrialization: Zhou Yu
 
-
    </p>
 
-
    <p>iGEM Club establishment in Nanjing University: Yang Zhanzhao, Wen Yuqi</p>
 
</div>
</div>

Revision as of 11:41, 16 October 2014

Human Practice

1. Meetup at Huazhong Agricultural University

On 23rd and 24th of August, we participated in a sharing conference established by Huazhong Agricultural University. Zhou Yu, Qin Zhen, Rong Hui and Zhang Qiuhao from our team went to Wuhan and met up with students from Peking University, Sun Yat-Sen University, Wuhan University and some other universities. We gave a presentation about our project and also got to know what other teams were busy with. An internet-based group was set up for further discussion. Another team from Wuhan focuses on the fatty acid as well, thus our two teams shared experience for a mutual progress. Although some of the team’s project is quite different from ours, their experience about problems solving is still valuable to us.

2. Internship in Qingdao Institute of Bioenergy and Bioprocess Technology, CAS

In late August, 3 members of our team, Zhou Yu, Rao Bing and Zhu Ruichi, went to Qingdao Institute of Bioenergy and Bioprocess Technology for the function test of the plasmids we built. They conducted a few experiments there including extracting fatty acids and examine the species of fatty acids under the guidance of the PhD students there. One of the significant experiment they did there was they applied fermenter to cultivate a huge amount of E.coli for more quantity of fatty acids.

3. Small-scale Industrialization

After the experiments in Qingdao Institute of Bioenergy and Bioprocess Technology, Zhou Yu went to the factory in Qingdao with our project. He tried to apply what we studied to practice. The technician taught him some experience on fermentation. At the same time, he also learned something from the industry and managed to apply to our project. Huge fermenters were used for fermentation in a large scale.

4. Establishment of iGEM Club in Nanjing University

A club named iGEM was formed by our team and another team ’Nanjing-China’ in our campus. We can publicize the idea of the iGEM competition to other students and thus it can attract more students to join this competition. Furthermore, this club intends to recruit some members from other departments and our team can therefore be more versatile. Anyway, the establishment of this club will enable the iGEM competition to continue at our university in the future.