Team:UST Beijing/Parts

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     <p align="left">PR, an abbreviation for proteorhodopsin, is a transmembrane protein which was discovered in 2000 through shortgun genome sequencing of unculturable bacteria isolated from the seawater off the coast of California. PR is a light-activated proton pump and generates a pmf which is short for proton motive force. This part was constructed as below: We replaced the PR's precusor sequence with the leader peptide of human cytochrome oxidase subunit 4 isoform 1, targeting it to mitochondrial inner membrane. Meanwhile, all the codons were re-designed according to codon usage bias for Homo sapiens.</p></div>
     <p align="left">PR, an abbreviation for proteorhodopsin, is a transmembrane protein which was discovered in 2000 through shortgun genome sequencing of unculturable bacteria isolated from the seawater off the coast of California. PR is a light-activated proton pump and generates a pmf which is short for proton motive force. This part was constructed as below: We replaced the PR's precusor sequence with the leader peptide of human cytochrome oxidase subunit 4 isoform 1, targeting it to mitochondrial inner membrane. Meanwhile, all the codons were re-designed according to codon usage bias for Homo sapiens.</p></div>
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    <p align="center">A new Biobrick device: Mitochondrial proteorhodopsin (humanized genetic code bias)<br>Partsregistry submission：<br>BBa_K603000 (pSB1AC3)<br>BBa_K603001 (pSB1C3)</p></div>
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Revision as of 11:24, 16 October 2014

USTB iGEM14 Parts

Parts We Used

Mitochondrial proteorhodopsin (humanized genetic code bias)

PR, an abbreviation for proteorhodopsin, is a transmembrane protein which was discovered in 2000 through shortgun genome sequencing of unculturable bacteria isolated from the seawater off the coast of California. PR is a light-activated proton pump and generates a pmf which is short for proton motive force. This part was constructed as below: We replaced the PR's precusor sequence with the leader peptide of human cytochrome oxidase subunit 4 isoform 1, targeting it to mitochondrial inner membrane. Meanwhile, all the codons were re-designed according to codon usage bias for Homo sapiens.

A new Biobrick device: Mitochondrial proteorhodopsin (humanized genetic code bias)
Partsregistry submission：
BBa_K603000 (pSB1AC3)
BBa_K603001 (pSB1C3)

Retrieved from "http://2014.igem.org/Team:UST_Beijing/Parts"