"
Team:NEFU China/Recycling
From 2014.igem.org
(Difference between revisions)
| Line 64: | Line 64: | ||
<p></p> | <p></p> | ||
<p>In order to verify the synthesis of the CdS nanocrystal in <em>E.coli</em> (<em>Rosetta plysS</em>), the bacteria was harvested by centrifuged in 3000*g for 3 min then suspended in resin and hardened at 60℃ for 16 hours.</p> | <p>In order to verify the synthesis of the CdS nanocrystal in <em>E.coli</em> (<em>Rosetta plysS</em>), the bacteria was harvested by centrifuged in 3000*g for 3 min then suspended in resin and hardened at 60℃ for 16 hours.</p> | ||
| - | <p>Then the hard pellets were cut into 60nm thin slices and the slices were floated on water and deposited on a carbon-coated copper TEM grid. Microscopy was performed with a | + | <p>Then the hard pellets were cut into 60nm thin slices and the slices were floated on water and deposited on a carbon-coated copper TEM grid. Microscopy was performed with a JEM-1400 microscope at 120-keV electron energy.</p> |
<h2 class="subtitle">Reference.</h2> | <h2 class="subtitle">Reference.</h2> | ||
| - | < | + | <ol class="refrence"> |
| - | < | + | <li>Damage of the Bacterial Cell Envelope by Antimicrobial Peptides Gramicidin S and PGLa as Revealed by Transmission and Scanning Electron Microscopy Antimicrob Agents Chemother. Aug 2010; 54(8): 3132–3142.</li> |
| - | Journal of biotechnology 153(2011) 125-132</ | + | <li>Biosynthesis and characterization of CdS quantum dots in genetically engineered Escherichia coli. |
| - | < | + | Journal of biotechnology 153(2011) 125-132</li> |
| + | <li>Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. Journal of Colloid and Interface Science 275 (2004) 177–182</li> | ||
| + | </ol> | ||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 09:18, 16 October 2014
Recycling
TEM Protocol.
In order to verify the synthesis of the CdS nanocrystal in E.coli (Rosetta plysS), the bacteria was harvested by centrifuged in 3000*g for 3 min then suspended in resin and hardened at 60℃ for 16 hours.
Then the hard pellets were cut into 60nm thin slices and the slices were floated on water and deposited on a carbon-coated copper TEM grid. Microscopy was performed with a JEM-1400 microscope at 120-keV electron energy.
Reference.
- Damage of the Bacterial Cell Envelope by Antimicrobial Peptides Gramicidin S and PGLa as Revealed by Transmission and Scanning Electron Microscopy Antimicrob Agents Chemother. Aug 2010; 54(8): 3132–3142.
- Biosynthesis and characterization of CdS quantum dots in genetically engineered Escherichia coli. Journal of biotechnology 153(2011) 125-132
- Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. Journal of Colloid and Interface Science 275 (2004) 177–182
