Team:KAIT JAPAN

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Welcome to KAIT JAPAN !

Our team started a new project !

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9/1 Team OPEN!

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[http://sites.google.com/site/kaitjapan/ KAIT Japan(Japanese)]

KAIT Japan

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Project:Abstract

~Cure allergy system~

Acquired immune system is one of two main immunity strategies in vertebrates. In this system , Helper T cells work mainly. Th1 cells and Tｈ2 cells keep balance mutually, and two cells usually control immune response. When this balance collapses and became the Th2 predominance, IL-5 is secreted excessively from Th2, and eosonophil,basophil,and mast cells are activated by IL-5. These activated cells secrete inflammatory materials that cause allergic symptoms such as bronchial asthma and atopic dermatitis. On the other hand, by stimulation of IL-12, naïve T cell differentiates into Th1 cell and Th1 cell alleviates allergic symptoms. We though it is possible to ameliorate allergic symptoms by decreasing IL-5 and increasing IL-12. Focusing on ligand dependent dimerization of IL-5 receptor ,we try to make IL5 independent expression system .

1. Expression of IL-10 receptor to E.coli.

2. Phosphorylation of STAT3.

3. Preparation of gene array with HlyA and L-12 promoter and receiving the STAT3.

4. Preparation of gene array with TolC and HlyB and HlyD promoter and to receive the STAT3.

@@ Line 84: / Line 84: @@
 '''[[Team:KAIT_Japan/Project|Project]]:<span style="color:#f33">Abstract'''</span>
 <br>
-:<big>Hay fever curE.coli ~revenge~</big>
+:<big>~Cure allergy system~</big>
 <br>
-:I looked back at the failure of last year. I think it tries to complete this plan this year. Japanese on of six people is troubled now by hay fever. These people take a medicine for its. But, If they take it , they become sleepy. So we are working on a project to relive hay fever by E.Coli to improve these. Mechanism of hay fever When an allergen invades it in the living body, naïve T cell differentiates in Th2. There is more Th2 than Th1, and the mast cell and others that is humoral immunity become active, and inflammation is in this way caused. We perform following four this time. ①Expression of IL-10 receptor to E.coli. ②Phosphorylation of STAT3. ③Preparation of gene array with HlyA and L-12 promoter and receiving the STAT3. ④Preparation of gene array with TolC and HlyB and HlyD promoter and to receive the STAT3.
+Acquired immune system is one of two main immunity strategies in vertebrates.
+In this system , Helper T cells work mainly.
+Th1 cells and Tｈ2 cells keep balance mutually, and two cells usually control immune response. When this balance collapses and became the Th2 predominance, IL-5 is secreted excessively from Th2, and eosonophil,basophil,and mast cells are activated by IL-5. These activated cells secrete inflammatory materials that cause allergic symptoms such as bronchial asthma and atopic dermatitis. On the other hand, by stimulation of IL-12, naïve T cell differentiates into Th1 cell and Th1 cell alleviates allergic symptoms. We though it is possible to ameliorate allergic symptoms by decreasing IL-5 and increasing IL-12. Focusing on ligand dependent dimerization of IL-5 receptor ,we try to make IL5 independent expression system .
 <P>